Recombinant Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FADD (phospho S194) antibody [EPR1816Y]
See all FADD primary antibodies -
Description
Rabbit monoclonal [EPR1816Y] to FADD (phospho S194) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FADD aa 150-250 (phospho S194). The exact sequence is proprietary.
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Positive control
- Jurkat cell lysate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1816Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab68475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP |
WB: 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).
Is unsuitable for Flow Cyt, ICC/IF or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. -
Sequence similarities
Contains 1 death domain.
Contains 1 DED (death effector) domain. -
Domain
Contains a death domain involved in the binding of the corresponding domain within Fas receptor. - Information by UniProt
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Database links
- Entrez Gene: 8772 Human
- Omim: 602457 Human
- SwissProt: Q13158 Human
- Unigene: 86131 Human
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Alternative names
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
see all
Images
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![Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)](https://www.abcam.com/ps/products/68/ab68475/Images/ab68475-82769-anti-fadd-phospho-s194-antibody-epr1816y-western-blot.jpg)
Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution
Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Datasheets and documents
References
ab68475 has not yet been referenced specifically in any publications.
