Recombinant Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-FADD (phospho S194) antibody [EPR1816Y]
See all FADD primary antibodies -
Description
Rabbit monoclonal [EPR1816Y] to FADD (phospho S194) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FADD aa 150-250 (phospho S194). The exact sequence is proprietary.
-
Positive control
- Jurkat cell lysate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1816Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Notes |
---|
WB: 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).
Is unsuitable for Flow Cyt, ICC/IF or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. -
Sequence similarities
Contains 1 death domain.
Contains 1 DED (death effector) domain. -
Domain
Contains a death domain involved in the binding of the corresponding domain within Fas receptor. - Information by UniProt
-
Database links
- Entrez Gene: 8772 Human
- Omim: 602457 Human
- SwissProt: Q13158 Human
- Unigene: 86131 Human
-
Alternative names
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
see all
Images
-
All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution
Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
Datasheet download
References (0)
ab68475 has not yet been referenced specifically in any publications.