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  1. Link

    fadd-phospho-s194-antibody-epr1816y-ab68475.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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RecombinantRabMAb

Recombinant Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)

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Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
  • Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-FADD (phospho S194) antibody [EPR1816Y]
    See all FADD primary antibodies
  • Description

    Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FADD aa 150-250 (phospho S194). The exact sequence is proprietary.

  • Positive control

    • Jurkat cell lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1816Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • FADD
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • FADD
    • Cancer
    • Cell Death
    • Necroptosis

Associated products

  • Alternative Versions

    • Anti-FADD (phospho S194) antibody [EPR1816Y] - BSA and Azide free (ab232047)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
  • Recombinant Protein

    • Recombinant Human FADD protein (ab89242)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Notes
Application notes
IP: 1/30.
WB: 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).

Is unsuitable for Flow Cyt, ICC/IF or IHC-P.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Function

    Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
  • Tissue specificity

    Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
  • Sequence similarities

    Contains 1 death domain.
    Contains 1 DED (death effector) domain.
  • Domain

    Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Target information above from: UniProt accession Q13158 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8772 Human
    • Omim: 602457 Human
    • SwissProt: Q13158 Human
    • Unigene: 86131 Human
    • Alternative names

      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all

    Images

    • Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
      Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
      All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution

      Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 23 kDa
      Observed band size: 27 kDa why is the actual band size different from the predicted?

    • Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
      Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab68475? Please let us know so that we can cite the reference in this datasheet.

    ab68475 has not yet been referenced specifically in any publications.

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