Recombinant
RabMAb

Recombinant Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-FADD (phospho S194) antibody [EPR1816Y]
    See all FADD primary antibodies

  • Description

    Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human FADD aa 150-250 (phospho S194). The exact sequence is proprietary.

  • Positive control

    • Jurkat cell lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab68475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/30.
    WB: 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).

    Is unsuitable for Flow Cyt, ICC/IF or IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.

    • Tissue specificity

      Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

    • Sequence similarities

      Contains 1 death domain.
      Contains 1 DED (death effector) domain.

    • Domain

      Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt

    • Database links

      • Alternative names

        • FADD antibody
        • FADD protein antibody
        • FADD_HUMAN antibody
        • Fas (TNFRSF6) associated via death domain antibody
        • Fas associated via death domain antibody
        • Fas associating death domain containing protein antibody
        • Fas associating protein antibody
        • Fas associating protein with death domain antibody
        • Fas TNFRSF6 associated via death domain antibody
        • FAS-associated death domain protein antibody
        • FAS-associating death domain-containing protein antibody
        • GIG 3 antibody
        • GIG3 antibody
        • Growth inhibiting gene 3 protein antibody
        • Growth-inhibiting gene 3 protein antibody
        • H sapiens mRNA for mediator of receptor induced toxicity antibody
        • Mediator of receptor induced toxicity antibody
        • MGC8528 antibody
        • MORT 1 antibody
        • MORT1 antibody
        • Protein FADD antibody
        see all

      Images

      • Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
        Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
        All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution

        Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
        Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution

        Predicted band size: 23 kDa
        Observed band size: 27 kDa
        why is the actual band size different from the predicted?

      References

      Publishing research using ab68475? Please let us know so that we can cite the reference in this datasheet.

      ab68475 has not yet been referenced specifically in any publications.

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