Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1816Y] to FADD (phospho S194) - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-FADD (phospho S194) antibody [EPR1816Y] - BSA and Azide free
See all FADD primary antibodies
DescriptionRabbit monoclonal [EPR1816Y] to FADD (phospho S194) - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human FADD aa 150-250. The exact sequence is proprietary.
- WB: Jurkat cells treated with calyculin A.
ab232047 is the carrier-free version of ab68475 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232047 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab232047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Sequence similaritiesContains 1 death domain.
Contains 1 DED (death effector) domain.
DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
- Information by UniProt
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution
Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68475).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232047 has not yet been referenced specifically in any publications.