Recombinant
RabMAb

Recombinant Anti-FADD (phospho S194) antibody [EPR1816Y] - BSA and Azide free (ab232047)

Overview

  • Product name

    Anti-FADD (phospho S194) antibody [EPR1816Y] - BSA and Azide free
    See all FADD primary antibodies
  • Description

    Rabbit monoclonal [EPR1816Y] to FADD (phospho S194) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FADD aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat cells treated with calyculin A.
  • General notes

    Ab232047 is the carrier-free version of ab68475. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232047 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
  • Tissue specificity

    Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
  • Sequence similarities

    Contains 1 death domain.
    Contains 1 DED (death effector) domain.
  • Domain

    Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt
  • Database links

  • Alternative names

    • FADD antibody
    • FADD protein antibody
    • FADD_HUMAN antibody
    • Fas (TNFRSF6) associated via death domain antibody
    • Fas associated via death domain antibody
    • Fas associating death domain containing protein antibody
    • Fas associating protein antibody
    • Fas associating protein with death domain antibody
    • Fas TNFRSF6 associated via death domain antibody
    • FAS-associated death domain protein antibody
    • FAS-associating death domain-containing protein antibody
    • GIG 3 antibody
    • GIG3 antibody
    • Growth inhibiting gene 3 protein antibody
    • Growth-inhibiting gene 3 protein antibody
    • H sapiens mRNA for mediator of receptor induced toxicity antibody
    • Mediator of receptor induced toxicity antibody
    • MGC8528 antibody
    • MORT 1 antibody
    • MORT1 antibody
    • Protein FADD antibody
    see all

Images

  • All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution

    Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 23 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68475).

References

ab232047 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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