Product nameAnti-FADD (phospho S194) antibody [EPR1816Y] - BSA and Azide free
See all FADD primary antibodies
DescriptionRabbit monoclonal [EPR1816Y] to FADD (phospho S194) - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human FADD aa 150-250. The exact sequence is proprietary.
- WB: Jurkat cells treated with calyculin A.
Ab232047 is the carrier-free version of ab68475. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232047 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab232047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Sequence similaritiesContains 1 death domain.
Contains 1 DED (death effector) domain.
DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
- Information by UniProt
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution
Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68475).
ab232047 has not yet been referenced specifically in any publications.