Overview

  • Product name

    Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield
    See all Fluoroshield reagents
  • Description

    Anti-Fade Fluorescence Mounting Medium
  • General notes

    Anti-Fade Fluorescence Mounting Medium ab104135 (previously called Fluoroshield Mounting Medium) is an aqueous mounting medium designed to preserve fluorescence when imaging tissues and cell samples.

    The formulation prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, R-Phycoerythrin (R-PE), Phyocyanin (PC), and Allophycocyanin (APC).

    Fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC.

    Mounting media products

    For fluorescent cell and tissue staining, Abcam recommends this product (Fluorescence Mounting Medium ab104135), Mounting Medium with DAPI ab104139, and Mounting Medium with PI ab104129. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI ab188804.

    For chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend aqueous Mounting Medium ab64230, or organic Limonene Mounting Medium ab104141


    Procedure

    - Bring the vial to room temperature.

    - Rinse slide to be mounted with distilled or deionized, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.

    - Turn the vial upside down and open the dropper to remove any air bubbles.

    - Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.

    - Let stand at room temperature for about 5 minutes.

    - Apply cover slip carefully avoiding air bubbles.

    - The specimen is ready for visualization under a microscope.

    Seal the edges of cover slip with nail polish, any organic medium or Limonene mounting medium ab104141. If a coverslip is not sealed, air bubbles will appear in a few days.

    Store the slide in the dark at 2-8°C.

    Removal of Coverslip Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deiononized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.

Properties

References

This product has been referenced in:

  • Dioli C  et al. Chronic stress triggers divergent dendritic alterations in immature neurons of the adult hippocampus, depending on their ultimate terminal fields. Transl Psychiatry 9:143 (2019). Read more (PubMed: 31028242) »
  • Castelucci BG  et al. Time-dependent regulation of morphological changes and cartilage differentiation markers in the mouse pubic symphysis during pregnancy and postpartum recovery. PLoS One 13:e0195304 (2018). IHC-Fr . Read more (PubMed: 29621303) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer



We do not have specific use by dates for most our products. If stored correctly, the products remain suitable for use for months and even for years.

However, we do strongly suggest to store the products as recommended on their respective datasheet and if no other information is given the product is stable for at least one year from teh date of purchase.

I can confirm that ab104135 (Fluoroshield Mounting Medium (20ml)) is stable and covered by our guarantee when stored at +4°C in the dark for one year.

https://www.abcam.com/abpromise

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Answer

Thank you for contacting Abcam.

For ab104135 the refractive index is 1.364 ± 0.002.

If there is anything else I can help you with, please let me know.

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Question
Answer

Thank you for your inquiry. The refractive index of Fluoroshield Mounting Medium is: 1.364 ± 0.002 Please note that these numbers apply to these mounting mediums in solution. Refractive indexes change when the water solvent evaporates and mounting media dries on slides. We do not have the means to measure the refractive indexes of dry mounting mediums; however we expect the numbers to go higher when dried. The refractive index of water is 1.3330. If you are using other Fluoroshield Mounting Media (e.g. with DAPI), we also have the refractive index available on request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any further advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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