Product nameAnti-FADS1 antibody [EPR6898]
See all FADS1 primary antibodies
DescriptionRabbit monoclonal [EPR6898] to FADS1
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human FADS1 aa 100-200. The exact sequence is proprietary.
- HepG2, Human fetal lung, fetal liver and fetal brain lysates; Human heart tissue; A549 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 7.20 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab126706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionComponent of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively.
Tissue specificityExpressed in many tissues, it is most abundant in the liver, brain, adrenal gland and heart. Found as well in skeletal muscle, lung, placenta, kidney, pancreas and retina.
PathwayLipid metabolism; polyunsaturated fatty acid biosynthesis.
Sequence similaritiesBelongs to the fatty acid desaturase family.
Contains 1 cytochrome b5 heme-binding domain.
Developmental stageHighly expressed in fetal liver and fetal brain.
DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- D5D antibody
- Delta 5 desaturase antibody
- Delta 5 fatty acid desaturase antibody
All lanes : Anti-FADS1 antibody [EPR6898] (ab126706) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal brain lysate
Lane 4 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Overlay histogram showing HepG2 cells stained with ab126706 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126706, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab126706, at 1/100 dilution, staining FADS1 in Paraffin-embedded Human heart tissue by Immunohistochemistry.
ab126706, at 1/100 dilution, staining FADS1 in A549 cells by Immunofluorescence.
Equilibrium disassociation constant (KD)
Learn more about KD
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This product has been referenced in:
- Sarr O et al. Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency. J Nutr Biochem 63:140-149 (2019). Read more (PubMed: 30368227) »
- Yang X et al. Dihomo-?-linolenic acid inhibits growth of xenograft tumors in mice bearing human pancreatic cancer cells (BxPC-3) transfected with delta-5-desaturase shRNA. Redox Biol 20:236-246 (2019). Read more (PubMed: 30384258) »