Product nameAnti-FADS2 antibody
See all FADS2 primary antibodies
DescriptionRabbit polyclonal to FADS2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey, Baboon, Orangutan
Synthetic peptide corresponding to Human FADS2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: Human liver tissue lysate; Human brain tissue lysates; HeLa whole cell lysate; Hep G2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab72189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 52 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionComponent of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.
Tissue specificityExpressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index.
PathwayLipid metabolism; polyunsaturated fatty acid biosynthesis.
Sequence similaritiesBelongs to the fatty acid desaturase family.
Contains 1 cytochrome b5 heme-binding domain.
Developmental stageFound in fetal heart.
DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- D6D antibody
- Delta(6) desaturase antibody
- Delta(6) fatty acid desaturase antibody
FADS2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to FADS2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72189.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
All lanes : Anti-FADS2 antibody (ab72189) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 62.5 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab72189 detects a strong band at 45 kDa in human liver and brain tissue lysates, aswell as hela and hepG2 whole cell lysates. This antibody was raised against an immunogen that is predicted to cross react with all known isoforms of Fatty acid desaturase 2 (FADS2). FADS2 has three isoforms, isoform one has a molecular weight of 52 kDa with isoforms two and three a molecular weight of 49 and 45 respectively(SwissProt data). The band we have observed is thought to represent isoform 3 of FADS2 (45 kDa).
This product has been referenced in:
- Naudí A et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . Read more (PubMed: 28185952) »