Overview

  • Product name

  • Description

    Rabbit polyclonal to FADS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey, Baboon, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human FADS2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90992)

  • Positive control

    • This antibody gave a positive signal in the following lysates: Human liver tissue lysate; Human brain tissue lysates; HeLa whole cell lysate; Hep G2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 52 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.
  • Tissue specificity

    Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index.
  • Pathway

    Lipid metabolism; polyunsaturated fatty acid biosynthesis.
  • Sequence similarities

    Belongs to the fatty acid desaturase family.
    Contains 1 cytochrome b5 heme-binding domain.
  • Developmental stage

    Found in fetal heart.
  • Domain

    The histidine box domains may contain the active site and/or be involved in metal ion binding.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • D6D antibody
    • Delta(6) desaturase antibody
    • Delta(6) fatty acid desaturase antibody
    • Delta-6 desaturase antibody
    • DES 6 antibody
    • DES6 antibody
    • FADS 2 antibody
    • FADS2 antibody
    • FADS2_HUMAN antibody
    • FADSD 6 antibody
    • FADSD6 antibody
    • Fatty acid desaturase 2 antibody
    • linoleoyl-CoA desaturase (delta-6-desaturase) like 2 antibody
    • LLCDL 2 antibody
    • LLCDL2 antibody
    • SLL0262 antibody
    • TU 13 antibody
    • TU13 antibody
    see all

Images

  • FADS2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to FADS2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72189.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 45kDa:FADS2.
  • All lanes : Anti-FADS2 antibody (ab72189) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 62.5 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    ab72189 detects a strong band at 45 kDa in human liver and brain tissue lysates, aswell as hela and hepG2 whole cell lysates. This antibody was raised against an immunogen that is predicted to cross react with all known isoforms of Fatty acid desaturase 2 (FADS2). FADS2 has three isoforms, isoform one has a molecular weight of 52 kDa with isoforms two and three a molecular weight of 49 and 45 respectively(SwissProt data). The band we have observed is thought to represent isoform 3 of FADS2 (45 kDa).

References

This product has been referenced in:

  • Naudí A  et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . Read more (PubMed: 28185952) »
See 1 Publication for this product

Customer reviews and Q&As

Answer



We have in our catalog one anti-FADS2, the reference ishttps://www.abcam.com/ab72189.Unfortunately the homology between the immunogen peptide and therainbow trout FADS2 is only 27% (see attachment). It is therefore veryunlikely this antibody is suitable for the study of FADS2 in fish.

I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The link is: http://www.biocompare.com

I would also like to recommend checking our range of antibodies that have been tested in Zebra fish, they may be suitable for your research work : https://www.abcam.com/zebrafish

Read More

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