Overview

  • Product name
    Anti-FAK antibody [EP695Y]
    See all FAK primary antibodies
  • Description
    Rabbit monoclonal [EP695Y] to FAK
  • Host species
    Rabbit
  • Specificity

    ab40794 recognises Focal adhesion kinase (FAK).

    The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow.

  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide within Human FAK aa 700-800. The exact sequence is proprietary.
    (Peptide available as ab211922)

  • Positive control
    • WB: NIH/3T3, Rat brain, HeLa, K-562 lysates IHC-P: human hepatocellular carcinoma
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250.

The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow.

See IHC antigen retrieval protocols.

WB 1/2000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa).Can be blocked with Human FAK peptide (ab211922).

For unpurified use at 1/1000

Target

  • Function
    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • Tissue specificity
    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Domain
    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • Post-translational
    modifications
    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • Cellular localization
    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • Database links
  • Alternative names
    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling FAK with purified ab40794 at 1:250 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Lane 1 : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution (Purified)
    Lane 2 : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 119 kDa
    Observed band size: 119 kDa

  • Lane 1 : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution (Purified)
    Lane 2 : Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 119 kDa
    Observed band size: 119 kDa

  • Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution + Hela cell lysate

    Predicted band size: 119 kDa
    Observed band size: 119 kDa

  • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794.
  • The image shows FAK antibody (ab40794) in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes.

    See Abreview

  • All lanes : Anti-FAK antibody [EP695Y] (ab40794)

    All lanes : Milk PBS Tween

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit poly clonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 119 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    Western blot analysis of RAW264.7 cells lysate (40μg/lane) labelling FAK with ab40794 at 1/5000 in 5% Milk PBS Tween for 16 hours at 4ºC. A HRP conjugated goat anti-rabbit poly clonal (1/5000) was used as the secondary antibody.

     

    See Abreview

References

This product has been referenced in:
  • Seok J  et al. The oncometabolite d-2-hydroxyglutarate induces angiogenic activity through the vascular endothelial growth factor receptor 2 signaling pathway. Int J Oncol 54:753-763 (2019). Read more (PubMed: 30483760) »
  • Zhang Y  et al. Chinese Herbal Medicine Wenxia Changfu Formula Reverses Cell Adhesion-Mediated Drug Resistance via the Integrin ß1-PI3K-AKT Pathway in Lung Cancer. J Cancer 10:293-304 (2019). Read more (PubMed: 30719123) »
See all 56 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (RAW264.7 cells)
Gel Running Conditions
Reduced Denaturing (Gradient 4 -20%)
Loading amount
40 µg
Specification
RAW264.7 cells
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Magali Boissiere

Verified customer

Submitted Feb 23 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH8.6, 100C, 20min
Sample
Human Tissue sections (placenta)
Specification
placenta
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (MDBK)
Specification
MDBK
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton in PBS (20 min)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Nov 05 2012

Application
Western blot
Sample
Cow Cell lysate - whole cell (MDBK)
Loading amount
5 µg
Specification
MDBK
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Nov 02 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (hippocampus)
Loading amount
25 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (6%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 31 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil: reactive)
Specification
Tonsil: reactive
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Jul 19 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cor 1 cells - unstimulated)
Specification
Cor 1 cells - unstimulated
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% TWEEN 20
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C

Mr. Carl Hobbs

Verified customer

Submitted Jul 19 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Mr. Carl Hobbs

Verified customer

Submitted Jul 19 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HUVEC)
Specification
HUVEC
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton X-100
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 20 2010

Question
Answer

Thank you for your enquiry. Unfortunately, the exact amino acid sequence information is proprietary and can not be released. However, I can provide you with a 50 amino acid sequence range from where the actual peptide sequence is located within, as listed below. T VSWDSGGSDE APPKPSRPGY PSPRSSEGFY PSPQHMVQTN HYQVSGYPGS, 700aa-750aa I hope this information will still be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

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