Recombinant Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP695Y] to FAK - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FAK antibody [EP695Y] - BSA and Azide free
See all FAK primary antibodies -
Description
Rabbit monoclonal [EP695Y] to FAK - BSA and Azide free -
Host species
Rabbit -
Specificity
ab271836 recognises Focal adhesion kinase (FAK).
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: human hepatocellular carcinoma
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General notes
ab271836 is the carrier-free version of ab40794.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP695Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)
- Alexa Fluor® 488 Anti-FAK antibody [EP695Y] (ab311051)
- Alexa Fluor® 647 Anti-FAK antibody [EP695Y] (ab311177)
- Alexa Fluor® 594 Anti-FAK antibody [EP695Y] (ab311832)
- Alexa Fluor® 568 Anti-FAK antibody [EP695Y] (ab313115)
- Alexa Fluor® 555 Anti-FAK antibody [EP695Y] (ab313308)
- Anti-FAK antibody [EP695Y] (ab40794)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 119 kDa.
For unpurified use at 1/1000
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IHC-P |
Use at an assay dependent concentration.
The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow. See IHC antigen retrieval protocols. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 119 kDa. For unpurified use at 1/1000
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IHC-P
Use at an assay dependent concentration. The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow. See IHC antigen retrieval protocols. |
Target
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Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
Tissue specificity
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
Domain
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
Post-translational
modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
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Database links
- Entrez Gene: 506270 Cow
- Entrez Gene: 5747 Human
- Entrez Gene: 14083 Mouse
- Entrez Gene: 25614 Rat
- Omim: 600758 Human
- SwissProt: Q05397 Human
- SwissProt: P34152 Mouse
- SwissProt: O35346 Rat
see all -
Alternative names
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
Images
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All lanes : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDaThis data was developed using ab40794, the same antibody clone in a different buffer formulation.
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All lanes : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDaThis data was developed using ab40794, the same antibody clone in a different buffer formulation.
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All lanes : Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : PTK2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 119 kDaThis data was developed using ab40794, the same antibody clone in a different buffer formulation
Lanes 1 - 4: Merged signal (red and green). Green - ab40794 observed at 119 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab40794 was shown to react with FAK in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255421 (knockout cell lysate ab263766) was used. Wild-type and FAK knockout samples were subjected to SDS-PAGE. ab40794 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling FAK with purified ab40794 at 1:250 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody.
Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40794).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab271836 has not yet been referenced specifically in any publications.