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  1. Link

    fak-phospho-s732-antibody-ab4792.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Integrins
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Anti-FAK (phospho S732) antibody (ab4792)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (15)

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Western blot - Anti-FAK (phospho S732) antibody (ab4792)
  • Western blot - Anti-FAK (phospho S732) antibody (ab4792)
  • Western blot - Anti-FAK (phospho S732) antibody (ab4792)

Key features and details

  • Rabbit polyclonal to FAK (phospho S732)
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FAK (phospho S732) antibody
    See all FAK primary antibodies
  • Description

    Rabbit polyclonal to FAK (phospho S732)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human FAK (phospho S732).

  • General notes


    Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that plays a key role in signalling by growth factors, extracellular matrix and stress signals. Indeed, Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Cyclin-dependent kinase-5 (Cdk5), a serine/threonine kinase and an important regulator in brain development, has been found to be essential for phosphorylation at serine 732. In the context of the Focal Adhesion Kinase-Related Non-Kinase (FRNK), the c-terminal non-kinase domain of Focal Adhesion Kinase that acts as a dominant negative inhibitor of Focal Adhesion Kinase signalling, other serine kinases appear to readily phosphorylate this same site. The functional significance of phosphorylation on serine 732 is yet to be determined.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 2.5% Glycerol, 0.1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phosphoserine, irrespective of the sequence. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at serine 732.
  • Primary antibody notes

    Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that plays a key role in signalling by growth factors, extracellular matrix and stress signals. Indeed, Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Cyclin-dependent kinase-5 (Cdk5), a serine/threonine kinase and an important regulator in brain development, has been found to be essential for phosphorylation at serine 732. In the context of the Focal Adhesion Kinase-Related Non-Kinase (FRNK), the c-terminal non-kinase domain of Focal Adhesion Kinase that acts as a dominant negative inhibitor of Focal Adhesion Kinase signalling, other serine kinases appear to readily phosphorylate this same site. The functional significance of phosphorylation on serine 732 is yet to be determined.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Integrins
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • FAK / PYK
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • FAK family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human FAK protein (ab105909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 125 kDa.
Notes
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 125 kDa.

Target

  • Function

    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • Tissue specificity

    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Domain

    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • Post-translational
    modifications

    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • Cellular localization

    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Target information above from: UniProt accession Q05397 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396416 Chicken
    • Entrez Gene: 5747 Human
    • Entrez Gene: 14083 Mouse
    • Entrez Gene: 399286 Xenopus laevis
    • Omim: 600758 Human
    • SwissProt: Q00944 Chicken
    • SwissProt: Q05397 Human
    • SwissProt: P34152 Mouse
    • SwissProt: Q91738 Xenopus laevis
    • Unigene: 395482 Human
    • Unigene: 254494 Mouse
    • Unigene: 6819 Xenopus laevis
    see all
  • Alternative names

    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Images

  • Western blot - Anti-FAK (phospho S732) antibody (ab4792)
    Western blot - Anti-FAK (phospho S732) antibody (ab4792)
    All lanes : Anti-Metabotropic Glutamate Receptor 6/MGLUR6 antibody (ab10314)

    Lane 1 : A549 without VEGF treatment.
    Lane 2 : A549 with 5 ng/ml VEGF treatment for 5 mins.

    Predicted band size: 125 kDa

  • Western blot - Anti-FAK (phospho S732) antibody (ab4792)
    Western blot - Anti-FAK (phospho S732) antibody (ab4792)

    Peptide Competition: Extracts prepared from cdk5 +/- mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with FAK pan antibody (1), or 0.34 µg/mL ab4792 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (2), the phosphopeptide corresponding to ab4792 (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method.

    Phosphatase Stripping: Extracts prepared from cdk5 +/- mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was treated with lambda phosphatase, then incubated with either FAK pan antibody (

  • Western blot - Anti-FAK (phospho S732) antibody (ab4792)
    Western blot - Anti-FAK (phospho S732) antibody (ab4792)
    Influence of cdk5:  Extracts prepared from cdk5 +/- (1, 3) and cdk5 -/- (2, 4) mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was incubated with either FAK pan antibody (1, 2), or with 0.34 µg/mL ab4792 antibody (3, 4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that cdk5 is essential for phosphorylation of FAK at serine 732. Other kinases appear to phosphorylate FRNK on this site. Influence of cdk5: Extracts prepared from cdk5 +/- (1, 3) and cdk5 -/- (2, 4) mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was incubated with either FAK pan antibody (1, 2), or with 0.34 µg/mL ab4792 antibody (3, 4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were de

Protocols

  • Peptide Competition Experiment
  • Western blotting protocol

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (15)

Publishing research using ab4792? Please let us know so that we can cite the reference in this datasheet.

ab4792 has been referenced in 15 publications.

  • Li X  et al. miR-365 secreted from M2 Macrophage-derived extracellular vesicles promotes pancreatic ductal adenocarcinoma progression through the BTG2/FAK/AKT axis. J Cell Mol Med 25:4671-4683 (2021). PubMed: 33811437
  • Li J  et al. Dynamic changes in cis-regulatory occupancy by Six1 and its cooperative interactions with distinct cofactors drive lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium. Nucleic Acids Res 48:2880-2896 (2020). PubMed: 31956913
  • Xue X  et al. HIF-1a interacts with Kindlin-2 and influences breast cancer elasticity: A study based on shear wave elastography imaging. Cancer Med 9:4971-4979 (2020). PubMed: 32436609
  • Ou L  et al. Sialylation of FGFR1 by ST6Gal-I overexpression contributes to ovarian cancer cell migration and chemoresistance. Mol Med Rep 21:1449-1460 (2020). PubMed: 32016470
  • Li J  et al. Pristimerin induces apoptosis and inhibits proliferation, migration in H1299 Lung Cancer Cells. J Cancer 11:6348-6355 (2020). PubMed: 33033518
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FAK (phospho S732) antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil - reactive)
Specification
Tonsil - reactive
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More

MR. Carl Hobbs

Verified customer

Submitted Sep 01 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FAK (phospho S732) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (e16 lung)
Specification
e16 lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More

MR. Carl Hobbs

Verified customer

Submitted Jul 19 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FAK (phospho S732) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Ovary)
Specification
Ovary
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Read More

MR. Carl Hobbs

Verified customer

Submitted Jun 08 2010

Immunocytochemistry/ Immunofluorescence abreview for Anti-FAK (phospho S732) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cor 1 cells)
Specification
Cor 1 cells
Fixative
Formaldehyde
Permeabilization
Yes - Tween 20
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Read More

MR. Carl Hobbs

Verified customer

Submitted Jun 08 2010

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