Anti-FAK (phospho S732) antibody (ab4792)
Key features and details
- Rabbit polyclonal to FAK (phospho S732)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-FAK (phospho S732) antibody
See all FAK primary antibodies -
Description
Rabbit polyclonal to FAK (phospho S732) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Xenopus laevis
-
Immunogen
Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains serine 732. The sequence is conserved in mouse, rat and chicken.
-
Positive control
- Cdk5 +/- and cdk5 -/- mouse brain lysates.
-
General notes
Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that plays a key role in signalling by growth factors, extracellular matrix and stress signals. Indeed, Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Cyclin-dependent kinase-5 (Cdk5), a serine/threonine kinase and an important regulator in brain development, has been found to be essential for phosphorylation at serine 732. In the context of the Focal Adhesion Kinase-Related Non-Kinase (FRNK), the c-terminal non-kinase domain of Focal Adhesion Kinase that acts as a dominant negative inhibitor of Focal Adhesion Kinase signalling, other serine kinases appear to readily phosphorylate this same site. The functional significance of phosphorylation on serine 732 is yet to be determined.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 2.5% Glycerol, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phosphoserine, irrespective of the sequence. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at serine 732. -
Primary antibody notes
Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that plays a key role in signalling by growth factors, extracellular matrix and stress signals. Indeed, Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Cyclin-dependent kinase-5 (Cdk5), a serine/threonine kinase and an important regulator in brain development, has been found to be essential for phosphorylation at serine 732. In the context of the Focal Adhesion Kinase-Related Non-Kinase (FRNK), the c-terminal non-kinase domain of Focal Adhesion Kinase that acts as a dominant negative inhibitor of Focal Adhesion Kinase signalling, other serine kinases appear to readily phosphorylate this same site. The functional significance of phosphorylation on serine 732 is yet to be determined. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab4792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 125 kDa. |
Target
-
Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
Tissue specificity
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
Domain
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
Post-translational
modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
-
Database links
- Entrez Gene: 396416 Chicken
- Entrez Gene: 5747 Human
- Entrez Gene: 14083 Mouse
- Entrez Gene: 25614 Rat
- Entrez Gene: 399286 Xenopus laevis
- Omim: 600758 Human
- SwissProt: Q00944 Chicken
- SwissProt: Q05397 Human
see all -
Alternative names
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
Images
-
Western blot - Anti-FAK (phospho S732) antibody (ab4792)All lanes : Anti-Metabotropic Glutamate Receptor 6/MGLUR6 antibody (ab10314)
Lane 1 : A549 without VEGF treatment.
Lane 2 : A549 with 5 ng/ml VEGF treatment for 5 mins.
Predicted band size: 125 kDa -
Western blot - Anti-FAK (phospho S732) antibody (ab4792)Peptide Competition: Extracts prepared from cdk5 +/- mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with FAK pan antibody (1), or 0.34
µ g/mL ab4792 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (2), the phosphopeptide corresponding to ab4792 (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method.Phosphatase Stripping: Extracts prepared from cdk5 +/- mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was treated with lambda phosphatase, then incubated with either FAK pan antibody (
-
Western blot - Anti-FAK (phospho S732) antibody (ab4792)Influence of cdk5: Extracts prepared from cdk5 +/- (1, 3) and cdk5 -/- (2, 4) mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was incubated with either FAK pan antibody (1, 2), or with 0.34µ g/mL ab4792 antibody (3, 4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that cdk5 is essential for phosphorylation of FAK at serine 732. Other kinases appear to phosphorylate FRNK on this site. Influence of cdk5: Extracts prepared from cdk5 +/- (1, 3) and cdk5 -/- (2, 4) mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was incubated with either FAK pan antibody (1, 2), or with 0.34 µg/mL ab4792 antibody (3, 4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were de
Protocols
References
ab4792 has been referenced in 8 publications.
- Li Y et al. Controlling the Multiscale Network Structure of Fibers To Stimulate Wound Matrix Rebuilding by Fibroblast Differentiation. ACS Appl Mater Interfaces 11:28377-28386 (2019). PubMed: 31251577
- Kour S et al. CDK5 Inhibitor Downregulates Mcl-1 and Sensitizes Pancreatic Cancer Cell Lines to Navitoclax. Mol Pharmacol 96:419-429 (2019). PubMed: 31467029
- Zhang X et al. Matrine inhibits the development and progression of ovarian cancer by repressing cancer associated phosphorylation signaling pathways. Cell Death Dis 10:770 (2019). PubMed: 31601793
- Gao Y et al. Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling. Commun Biol 2:359 (2019). PubMed: 31602408
- Jacobshagen M et al. The serotonin 6 receptor controls neuronal migration during corticogenesis via a ligand-independent Cdk5-dependent mechanism. Development 141:3370-7 (2014). WB ; Mouse . PubMed: 25078650
- Esencay M et al. CXCR7 is induced by hypoxia and mediates glioma cell migration towards SDF-1a. BMC Cancer 13:347 (2013). WB ; Human . PubMed: 23865743
- Lock FE et al. Differential regulation of adhesion complex turnover by ROCK1 and ROCK2. PLoS One 7:e31423 (2012). WB ; Human . PubMed: 22348083
- Zagzag D et al. Hypoxia- and vascular endothelial growth factor-induced stromal cell-derived factor-1alpha/CXCR4 expression in glioblastomas: one plausible explanation of Scherer's structures. Am J Pathol 173:545-60 (2008). WB ; Human . PubMed: 18599607
