Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Ovary)
Specification
Ovary
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Submitted composite image: upper image shows clear nuclear positivity in the middle secondary follicle....specific to mitotic cells. Caveat: I am not yet familiar with pFAK distribution pattern so I qualify my "excellent" rating.
Abcam response
Many thanks for your feedback on ab4792 (FAK (phospho S732) antibody) in IHC-P on mouse ovaries. We invite comments on the mitotic cell staining detected with this antibody.
Mr. Carl Hobbs
Verified customer
Submitted Jun 08 2010