Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FAK (phospho S732) antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (e16 lung)
Specification
e16 lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/300
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
A qualified "Excellent" Abreview rating.
Across several species, I obtain identical positivity restricted to mitotic bodies.
NB: to me, they appear to be mitotic bodies I may be wrong.
Submitted image of developing lung shows clear positivity in most mitotic bodies ( brown staining) but, not all ( red arrowheads).
Green arrowheads are irrelevant, indicating nucleated rbcs in blood vessels.

Mr. Carl Hobbs

Verified customer

Submitted Jul 19 2010

Sign up