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    fak-phospho-y397-antibody-m121-ab24781.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Integrins
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Anti-FAK (phospho Y397) antibody [M121] (ab24781)

  • Datasheet
Reviews (1)Q&A (1)References (5)

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Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)

    Key features and details

    • Mouse monoclonal [M121] to FAK (phospho Y397)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-FAK (phospho Y397) antibody [M121]
      See all FAK primary antibodies
    • Description

      Mouse monoclonal [M121] to FAK (phospho Y397)
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to Human FAK (phospho Y397) conjugated to keyhole limpet haemocyanin.

    • Positive control

      • Untreated HUVEC cells.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol, 0.1% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      M121
    • Isotype

      IgG1
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Integrins
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • FAK / PYK
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Focal Adhesions
      • Cancer
      • Signal transduction
      • Protein phosphorylation
      • Tyrosine kinases
      • FAK family

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant human FAK protein (ab105909)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab24781 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500. Detects a band of approximately 125 kDa.
    Notes
    WB
    1/500. Detects a band of approximately 125 kDa.

    Target

    • Function

      Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • Tissue specificity

      Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • Domain

      The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • Post-translational
      modifications

      Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • Cellular localization

      Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Target information above from: UniProt accession Q05397 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5747 Human
      • Omim: 600758 Human
      • SwissProt: Q05397 Human
      • Unigene: 395482 Human
      • Alternative names

        • FADK 1 antibody
        • FADK antibody
        • FAK related non kinase polypeptide antibody
        • FAK1 antibody
        • FAK1_HUMAN antibody
        • Focal adhesion kinase 1 antibody
        • Focal adhesion Kinase antibody
        • Focal adhesion kinase isoform FAK Del33 antibody
        • Focal adhesion kinase related nonkinase antibody
        • FRNK antibody
        • p125FAK antibody
        • pp125FAK antibody
        • PPP1R71 antibody
        • Protein phosphatase 1 regulatory subunit 71 antibody
        • Protein tyrosine kinase 2 antibody
        • Protein-tyrosine kinase 2 antibody
        • Ptk2 antibody
        • PTK2 protein tyrosine kinase 2 antibody
        see all

      Images

      • Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)
        Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)
        All lanes : Anti-FAK (phospho Y397) antibody [M121] (ab24781)

        Lane 1 : HUVEC untreated cells.
        Lane 2 : HUVEC cells treated with alkaline phosphatase

        Observed band size: 125 kDa why is the actual band size different from the predicted?

      Protocols

      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (5)

      Publishing research using ab24781? Please let us know so that we can cite the reference in this datasheet.

      ab24781 has been referenced in 5 publications.

      • Zhang Y  et al. LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer. J Cell Mol Med N/A:N/A (2020). PubMed: 32618099
      • Kamarajan P  et al. Periodontal pathogens promote cancer aggressivity via TLR/MyD88 triggered activation of Integrin/FAK signaling that is therapeutically reversible by a probiotic bacteriocin. PLoS Pathog 16:e1008881 (2020). PubMed: 33002094
      • Garcia-Keller C  et al. Relapse-Associated Transient Synaptic Potentiation Requires Integrin-Mediated Activation of Focal Adhesion Kinase and Cofilin in D1-Expressing Neurons. J Neurosci 40:8463-8477 (2020). PubMed: 33051346
      • Pucciarelli D  et al. Hypoxia increases the heterogeneity of melanoma cell populations and affects the response to vemurafenib. Mol Med Rep 13:3281-8 (2016). PubMed: 26936534
      • Babayan AH  et al. Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation. J Neurosci 32:12854-61 (2012). ICC/IF ; Rat . PubMed: 22973009

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-FAK (phospho Y397) antibody [M121]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Mouse Fibroblasts)
      Permeabilization
      Yes - Triton-X 100
      Specification
      Mouse Fibroblasts
      Blocking step
      Tween as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Hana Khoury

      Verified customer

      Submitted Jun 04 2020

      Question

      Please suggest a suitable positive control for IHC-P with ab24781

      Read More

      Abcam community

      Verified customer

      Asked on Sep 28 2012

      Answer

      Thank you for your telephone enquiry yesterday afternoon.

      As discussed on the telephone, I am sorry to confirm that as far as we are aware, ab24781 has never been tested in IHC-P. All tested applications covered by the 6 month guaranteeare specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

      With regards to a positive control for this applciation, the following page from the SwisProt./Uniprot protein database site indicates that FAK (phospho Y397) is quite ubiquitous:
      http://www.uniprot.org/uniprot/Q05397

      Our other antibody to FAK specific for the phospho Y397 residue has been used succesfully in brain tissue.

      I can recommend a literature search to obtain further details.

      As you are using this antibody in a new application, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

      Read More

      Abcam Scientific Support

      Answered on Sep 28 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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