Anti-FAK (phospho Y397) antibody [M121] (ab24781)
![Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)](https://www.abcam.com/ps/products/24/ab24781/Images/ab24781-35618-anti-fak-phospho-y397-antibody-m121-western-blot.jpg "Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)")
Key features and details
- Mouse monoclonal [M121] to FAK (phospho Y397)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-FAK (phospho Y397) antibody [M121]
See all FAK primary antibodies -
Description
Mouse monoclonal [M121] to FAK (phospho Y397) -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human FAK (phospho Y397) conjugated to keyhole limpet haemocyanin.
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Positive control
- Untreated HUVEC cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M121 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500. Detects a band of approximately 125 kDa.
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| Notes |
|---|
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WB
1/500. Detects a band of approximately 125 kDa. |
Target
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Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
Tissue specificity
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
Domain
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
Post-translational
modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
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Database links
- Entrez Gene: 5747 Human
- Omim: 600758 Human
- SwissProt: Q05397 Human
- Unigene: 395482 Human
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Alternative names
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
Images
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Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)All lanes : Anti-FAK (phospho Y397) antibody [M121] (ab24781)
Lane 1 : HUVEC untreated cells.
Lane 2 : HUVEC cells treated with alkaline phosphatase
Observed band size: 125 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab24781 has been referenced in 5 publications.
- Zhang Y et al. LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer. J Cell Mol Med N/A:N/A (2020). PubMed: 32618099
- Kamarajan P et al. Periodontal pathogens promote cancer aggressivity via TLR/MyD88 triggered activation of Integrin/FAK signaling that is therapeutically reversible by a probiotic bacteriocin. PLoS Pathog 16:e1008881 (2020). PubMed: 33002094
- Garcia-Keller C et al. Relapse-Associated Transient Synaptic Potentiation Requires Integrin-Mediated Activation of Focal Adhesion Kinase and Cofilin in D1-Expressing Neurons. J Neurosci 40:8463-8477 (2020). PubMed: 33051346
- Pucciarelli D et al. Hypoxia increases the heterogeneity of melanoma cell populations and affects the response to vemurafenib. Mol Med Rep 13:3281-8 (2016). PubMed: 26936534
- Babayan AH et al. Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation. J Neurosci 32:12854-61 (2012). ICC/IF ; Rat . PubMed: 22973009
