Anti-FAK (phospho Y397) antibody [M121] (ab24781)
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- Specific References (1)
- Protocols
Overview
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Product nameAnti-FAK (phospho Y397) antibody [M121]
See all FAK primary antibodies -
DescriptionMouse monoclonal [M121] to FAK (phospho Y397)
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 397 of human FAK. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse FAK.
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Positive control
- Untreated HUVEC cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberM121
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab24781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 22973009 | |
| WB | 1/500. Detects a band of approximately 125 kDa. | |
| ELISA | 1/2000. |
Target
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FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
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Tissue specificityExpressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
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Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
DomainThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
Post-translational
modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
Cellular localizationCell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
- Information by UniProt
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Database links
- Entrez Gene: 5747 Human
- Entrez Gene: 25614 Rat
- Omim: 600758 Human
- SwissProt: Q05397 Human
- SwissProt: O35346 Rat
- Unigene: 395482 Human
- Unigene: 2809 Rat
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Alternative names
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
Images
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![Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)](https://www.abcam.com/ps/products/24/ab24781/Images/ab24781_1.jpg)
Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)All lanes : Anti-FAK (phospho Y397) antibody [M121] (ab24781)
Lane 1 : HUVEC untreated cells.
Lane 2 : HUVEC cells treated with alkaline phosphatase
Observed band size: 125 kDa (why is the actual band size different from the predicted?)
References
This product has been referenced in:
- Babayan AH et al. Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation. J Neurosci 32:12854-61 (2012). ICC/IF ; Rat . Read more (PubMed: 22973009) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"