Anti-FAK (phospho Y397) antibody [M121] (ab24781)

Reviews (1)Q&A (1)References (2)

Key features and details

  • Mouse monoclonal [M121] to FAK (phospho Y397)
  • Suitable for: WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-FAK (phospho Y397) antibody [M121]
    See all FAK primary antibodies

  • Description

    Mouse monoclonal [M121] to FAK (phospho Y397)

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 397 of human FAK. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse FAK.

  • Positive control

    • Untreated HUVEC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab24781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 125 kDa.

Target

  • Function

    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

  • Tissue specificity

    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.

  • Domain

    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.

  • Post-translational
    modifications

    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.

  • Cellular localization

    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt

  • Database links

    • Alternative names

      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    Images

    • Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)
      Western blot - Anti-FAK (phospho Y397) antibody [M121] (ab24781)
      All lanes : Anti-FAK (phospho Y397) antibody [M121] (ab24781)

      Lane 1 : HUVEC untreated cells.
      Lane 2 : HUVEC cells treated with alkaline phosphatase

      Observed band size: 125 kDa
      why is the actual band size different from the predicted?

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab24781? Please let us know so that we can cite the reference in this datasheet.

    ab24781 has been referenced in 2 publications.

    • Pucciarelli D  et al. Hypoxia increases the heterogeneity of melanoma cell populations and affects the response to vemurafenib. Mol Med Rep 13:3281-8 (2016). PubMed: 26936534
    • Babayan AH  et al. Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation. J Neurosci 32:12854-61 (2012). ICC/IF ; Rat . PubMed: 22973009

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FAK (phospho Y397) antibody [M121]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse Fibroblasts)
    Permeabilization
    Yes - Triton-X 100
    Specification
    Mouse Fibroblasts
    Blocking step
    Tween as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Hana Khoury

    Verified customer

    Submitted Jun 04 2020

    Question

    Please suggest a suitable positive control for IHC-P with ab24781

    Read More
    Answer

    Thank you for your telephone enquiry yesterday afternoon.

    As discussed on the telephone, I am sorry to confirm that as far as we are aware, ab24781 has never been tested in IHC-P. All tested applications covered by the 6 month guaranteeare specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

    With regards to a positive control for this applciation, the following page from the SwisProt./Uniprot protein database site indicates that FAK (phospho Y397) is quite ubiquitous:
    http://www.uniprot.org/uniprot/Q05397

    Our other antibody to FAK specific for the phospho Y397 residue has been used succesfully in brain tissue.

    I can recommend a literature search to obtain further details.

    As you are using this antibody in a new application, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More

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