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Cardiovascular Angiogenesis Adhesion / ECM Integrins
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Anti-FAK (phospho Y407) antibody (ab4814)

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Western blot - Anti-FAK (phospho Y407) antibody (ab4814)

    Key features and details

    • Rabbit polyclonal to FAK (phospho Y407)
    • Suitable for: WB
    • Reacts with: Chicken
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human FAK protein (ab105909)
    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-FAK (phospho Y407) antibody
      See all FAK primary antibodies
    • Description

      Rabbit polyclonal to FAK (phospho Y407)
    • Host species

      Rabbit
    • Specificity

      Phosphorylation site-specific antibody selective for the phosphorylated form of the Focal Adhesion Kinase enzyme containing a phosphate on tyrosine 407. The antibody has been shown to recognize Focal Adhesion Kinase (approximately 125 kDa) in chick embryo fibroblast cells plated on fibronectin.
    • Tested Applications & Species

      Application Species
      WB
      Chicken
      See all applications and species data
    • Immunogen

      Synthetic peptide derived from the region of Focal Adhesion Kinase that contains tyrosine 407. The sequence is conserved in human, mouse, rat, chicken and frog.

    • General notes


      Focal Adhesion Kinase is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The activity of the phosphorylation site pTyr407 is currently unknown.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.3
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at tyrosine 407.
    • Primary antibody notes

      Focal Adhesion Kinase is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The activity of the phosphorylation site pTyr407 is currently unknown.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Integrins
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • FAK / PYK
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Focal Adhesions
      • Cancer
      • Signal transduction
      • Protein phosphorylation
      • Tyrosine kinases
      • FAK family

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human FAK protein (ab105909)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab4814 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Chicken
    Application Abreviews Notes
    WB
    1/1000. Predicted molecular weight: 125 kDa.
    Notes
    WB
    1/1000. Predicted molecular weight: 125 kDa.

    Target

    • Function

      Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • Tissue specificity

      Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • Domain

      The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • Post-translational
      modifications

      Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • Cellular localization

      Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Target information above from: UniProt accession Q05397 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 396416 Chicken
      • SwissProt: Q00944 Chicken
      • Alternative names

        • FADK 1 antibody
        • FADK antibody
        • FAK related non kinase polypeptide antibody
        • FAK1 antibody
        • FAK1_HUMAN antibody
        • Focal adhesion kinase 1 antibody
        • Focal adhesion Kinase antibody
        • Focal adhesion kinase isoform FAK Del33 antibody
        • Focal adhesion kinase related nonkinase antibody
        • FRNK antibody
        • p125FAK antibody
        • pp125FAK antibody
        • PPP1R71 antibody
        • Protein phosphatase 1 regulatory subunit 71 antibody
        • Protein tyrosine kinase 2 antibody
        • Protein-tyrosine kinase 2 antibody
        • Ptk2 antibody
        • PTK2 protein tyrosine kinase 2 antibody
        see all

      Images

      • Western blot - Anti-FAK (phospho Y407) antibody (ab4814)
        Western blot - Anti-FAK (phospho Y407) antibody (ab4814)
        Cell extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to nitrocellulose. Membranes were incubated with 0.3 µg/mL ab4814, following prior incubation in the absence (none) or presence of the peptide immunogen, the non-phosphopeptide corresponding to the FAK phosphopeptide, or a generic phosphotyrosine peptide. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, therefore demonstrating the specificity of ab4814 for this phosphorylated residue. Cell extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to

      Protocols

      • Peptide Competition Experiment
      • Western blotting protocol

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab4814? Please let us know so that we can cite the reference in this datasheet.

      ab4814 has not yet been referenced specifically in any publications.

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