Product nameAnti-FAK (phospho Y861) antibody
See all FAK primary antibodies
DescriptionRabbit polyclonal to FAK (phospho Y861)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Chicken, Human
Predicted to work with: Rat, Frog
Synthetic peptide corresponding to Human FAK (phospho Y861). A chemically synthesized phosphopeptide derived from the region of Human FAK containing tyrosine 861.
Database link: Q05397
- Primary chicken embryo fibroblasts (CEF) expressing FAK protein and plated on fibronectin, or A459 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, 49% PBS
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody negatively preadsorbed using a nonphosphopeptide.
Our Abpromise guarantee covers the use of ab200811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 119 kDa.|
FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Tissue specificityExpressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
DomainThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
Cellular localizationCell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
- Information by UniProt
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
All lanes : Anti-FAK (phospho Y861) antibody (ab200811) at 1/1000 dilution
Lane 1 : Extracts of primary CEF cells expressing FAK with phosphopeptide immunogen
Lane 2 : Extracts of primary CEF cells expressing FAK with a generic phosphotyrosine-containing peptide
Lane 3 : Extracts of primary CEF cells expressing FAK with a non-phosphorylated peptide corresponding to the immunogen
Lane 4 : Extracts of primary CEF cells expressing FAK
All lanes : Goat F(ab’)2 anti-rabbit IgG HRP
Developed using the ECL technique.
Predicted band size: 119 kDa
Only the phosphopeptide corresponding to FAK [pY861]
blocks the antibody signal (lane 1) demonstrating the
specificity of the antibody.
ab200811 has not yet been referenced specifically in any publications.