Overview

  • Product name

    Anti-FAK (phospho Y861) antibody
    See all FAK primary antibodies
  • Description

    Rabbit polyclonal to FAK (phospho Y861)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Chicken, Human
    Predicted to work with: Rat, Frog
  • Immunogen

    Synthetic peptide corresponding to Human FAK (phospho Y861). A chemically synthesized phosphopeptide derived from the region of Human FAK containing tyrosine 861.
    Database link: Q05397

  • Positive control

    • Primary chicken embryo fibroblasts (CEF) expressing FAK protein and plated on fibronectin, or A459 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab200811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 119 kDa.

Target

  • Function

    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • Tissue specificity

    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Domain

    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • Post-translational
    modifications

    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • Cellular localization

    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • Database links

  • Alternative names

    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Images

  • All lanes : Anti-FAK (phospho Y861) antibody (ab200811) at 1/1000 dilution

    Lane 1 : Extracts of primary CEF cells expressing FAK with phosphopeptide immunogen
    Lane 2 : Extracts of primary CEF cells expressing FAK with a generic phosphotyrosine-containing peptide
    Lane 3 : Extracts of primary CEF cells expressing FAK with a non-phosphorylated peptide corresponding to the immunogen
    Lane 4 : Extracts of primary CEF cells expressing FAK

    Secondary
    All lanes : Goat F(ab’)2 anti-rabbit IgG HRP

    Developed using the ECL technique.

    Predicted band size: 119 kDa



    Only the phosphopeptide corresponding to FAK [pY861]
    blocks the antibody signal (lane 1) demonstrating the
    specificity of the antibody.

References

ab200811 has not yet been referenced specifically in any publications.

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