Recombinant
RabMAb

Recombinant Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813)

Overview

  • Product name

    Anti-FAK (phospho Y925) antibody [EPR22330-75]
    See all FAK primary antibodies
  • Description

    Rabbit monoclonal [EPR22330-75] to FAK (phospho Y925)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FAK aa 900-1000 (phospho Y925). The exact sequence is proprietary.
    Database link: Q05397

  • Positive control

    • WB: HepG2 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab230813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 119 kDa.
Dot blot 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • Tissue specificity

      Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • Domain

      The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • Post-translational
      modifications

      Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • Cellular localization

      Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Information by UniProt
    • Database links

    • Alternative names

      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    Images

    • All lanes : Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813) at 1/1000 dilution

      Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate (Untreated membrane)
      Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate (Phosphatase treated membrane)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 119 kDa


      Exposure time: 70 seconds


      FAK can be cleaved at several sites. The molecular weight observed is consistent with what has been described in the literature (PMID: 10545505, 9642276).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Dot Blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813 at a 1/1000 dilution). Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as secondary antibody at 1/100000 dilution.

      Lane 1: FAK phospho Y925 peptide.

      Lane 2: FAK non-phospho peptide 

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 mins.

    References

    ab230813 has not yet been referenced specifically in any publications.

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