Overview

  • Product name

    Anti-FAM111A antibody [EPR14407]
    See all FAM111A primary antibodies
  • Description

    Rabbit monoclonal [EPR14407] to FAM111A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human FAM111A aa 1-150. The exact sequence is proprietary.
    Database link: Q96PZ2

  • Positive control

    • SW480, BxPC-3 or T47-D cell lysates; T47-D cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14407
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/100.

Target

  • Relevance

    This protein of unknown function belongs to the FAM111 family.
  • Database links

  • Alternative names

    • Family with sequence similarity 111 member A antibody
    • GCLEB antibody
    • Hypothetical protein LOC63901 antibody
    • KCS2 antibody
    • KIAA1895 antibody
    • Protein FAM111A antibody
    • RGD1560913 antibody
    see all

Images

  • All lanes : Anti-FAM111A antibody [EPR14407] (ab184572) at 1/1000 dilution

    Lane 1 : SW480 cell lysate
    Lane 2 : BxPC-3 cell lysate
    Lane 3 : T47-D cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed T47-D cells labeling FAM111A with ab184572 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/1200 dilution (red). Counter stained with Dapi (blue).

References

This product has been referenced in:

  • Panda D  et al. Triad of human cellular proteins, IRF2, FAM111A, and RFC3, restrict replication of orthopoxvirus SPI-1 host-range mutants. Proc Natl Acad Sci U S A 114:3720-3725 (2017). Read more (PubMed: 28320935) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184572.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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