Overview

  • Product name

    Anti-FAM114A1 antibody
  • Description

    Rabbit polyclonal to FAM114A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human FAM114A1 aa 141-200.
    Sequence:

    ASATVGHGLTAVKEKAGATLRIHGVNSGSSEGAQPNTENGVPEITDAATD QGPAESPPTS


    Database link: Q8IWE2

  • Positive control

    • ICC/IF: BJ cells. WB: RT4 cell lysate.
  • General notes

    Previously labelled as NXP20.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB 1/250 - 1/500. Predicted molecular weight: 61 kDa.

Target

  • Relevance

    FAM114A1 belongs to the FAM114 family and may play a role in neuronal cell development.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • 1190001N04Rik, antibody
    • 9130005N14Rik antibody
    • FAM114A1 antibody
    • FAM114A1 family with sequence similarity 114 member A1 antibody
    • Family with sequence similarity 114, member A1 antibody
    • Hypothetical protein LOC92689 antibody
    • MGC117924 antibody
    • nervous system over expressed protein 20 antibody
    • Nervous system over-expressed protein 20 antibody
    • nervous system overexpressed protein 20 antibody
    • Noxp20 antibody
    • NXP20 antibody
    • OTTMUSP00000027384 antibody
    • Protein NOXP20 antibody
    • RGD1560201 antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized BJ cells stained for FAM114A1 (green) using ab221806 at 4 µg/ml in ICC/IF.

  • Anti-FAM114A1 antibody (ab221806) at 1/250 dilution + RT4 (human urinary bladder cancer cell line) cell lysate

    Predicted band size: 61 kDa

References

ab221806 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Loading amount
30 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jun 15 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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