Anti-FAM120A/OSSA antibody (ab156695)
Key features and details
- Rabbit polyclonal to FAM120A/OSSA
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FAM120A/OSSA antibody
See all FAM120A/OSSA primary antibodies -
Description
Rabbit polyclonal to FAM120A/OSSA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
-
Immunogen
Synthetic peptide corresponding to Human FAM120A/OSSA aa 1068-1118.
Database link: NP_055427.2 -
Positive control
- 293T, Jurkat, NIH 3T3 and HeLa whole cell lysate (ab150035).
-
General notes
This product was previously labelled as FAM120A
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab156695 was affinity purified using an epitope specific to FAM120A/OSSA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Mouse
Human
|
| All applications |
Rat
Rabbit
Guinea pig
Hamster
Chimpanzee
Macaque monkey
Rhesus monkey
Gorilla
Common marmoset
Orangutan
Elephant
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2500. Predicted molecular weight: 121 kDa.
|
|
| IP |
Use at 2-10 µg/mg of lysate.
|
| Notes |
|---|
|
WB
1/500 - 1/2500. Predicted molecular weight: 121 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Function
May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses. -
Tissue specificity
Ubiquitously expressed. Highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa (at protein level). -
Sequence similarities
Belongs to the constitutive coactivator of PPAR-gamma family. -
Post-translational
modificationsArg-982 is dimethylated, probably to asymmetric dimethylarginine.
Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure. -
Cellular localization
Cytoplasm. Cell membrane. Translocates to plasma membrane upon ultraviolet exposure. - Information by UniProt
-
Database links
- Entrez Gene: 100398882 Common marmoset
- Entrez Gene: 23196 Human
- Entrez Gene: 218236 Mouse
- Entrez Gene: 291019 Rat
- GenBank: NP_055427.2 Human
- Omim: 612265 Human
- SwissProt: Q9NZB2 Human
- SwissProt: Q6A0A9 Mouse
see all -
Alternative names
- C9orf10 antibody
- Chromosome 9 open reading frame 10 antibody
- Constitutive coactivator of PPAR gamma like protein 1 antibody
see all
Images
-
Western blot - Anti-FAM120A/OSSA antibody (ab156695)All lanes : Anti-FAM120A/OSSA antibody (ab156695) at 1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Predicted band size: 121 kDa
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Immunoprecipitation - Anti-FAM120A/OSSA antibody (ab156695)Detection of FAM120A/OSSA by Western blot of immunoprecipitate.
Immunoprecipitation of FAM120A/OSSA (6 µg of antibody per mg of Jurkat whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: alternative rabbit anti-FAM120A/OSSA antibody
Lane 2: ab156695
Lane 3: Control IgG
For the subsequent Western blot of immunoprecipitates, ab156695 was used at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
References (1)
ab156695 has been referenced in 1 publication.
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708
