Product nameAnti-FAM120A/OSSA antibody
See all FAM120A/OSSA primary antibodies
DescriptionRabbit polyclonal to FAM120A/OSSA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Hamster, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Elephant
Synthetic peptide corresponding to Human FAM120A/OSSA aa 1068-1118.
Database link: NP_055427.2
- 293T, Jurkat, NIH 3T3 and HeLa whole cell lysate (ab150035).
This product was previously labelled as FAM120A
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab156695 was affinity purified using an epitope specific to FAM120A/OSSA immobilized on solid support.
Our Abpromise guarantee covers the use of ab156695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 121 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.
Tissue specificityUbiquitously expressed. Highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa (at protein level).
Sequence similaritiesBelongs to the constitutive coactivator of PPAR-gamma family.
modificationsArg-982 is dimethylated, probably to asymmetric dimethylarginine.
Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure.
Cellular localizationCytoplasm. Cell membrane. Translocates to plasma membrane upon ultraviolet exposure.
- Information by UniProt
- C9orf10 antibody
- Chromosome 9 open reading frame 10 antibody
- Constitutive coactivator of PPAR gamma like protein 1 antibody
All lanes : Anti-FAM120A/OSSA antibody (ab156695) at 1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Predicted band size: 121 kDa
Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of FAM120A/OSSA by Western blot of immunoprecipitate.
Immunoprecipitation of FAM120A/OSSA (6 µg of antibody per mg of Jurkat whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: alternative rabbit anti-FAM120A/OSSA antibody
Lane 2: ab156695
Lane 3: Control IgG
For the subsequent Western blot of immunoprecipitates, ab156695 was used at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.