Anti-FAM120A/OSSA antibody (ab156695)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-FAM120A/OSSA antibody
See all FAM120A/OSSA primary antibodies -
Description
Rabbit polyclonal to FAM120A/OSSA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Hamster, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human FAM120A/OSSA aa 1068-1118.
Database link: NP_055427.2 -
Positive control
- 293T, Jurkat, NIH 3T3 and HeLa whole cell lysate (ab150035).
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General notes
This product was previously labelled as FAM120A
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab156695 was affinity purified using an epitope specific to FAM120A/OSSA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab156695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2500. Predicted molecular weight: 121 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses. -
Tissue specificity
Ubiquitously expressed. Highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa (at protein level). -
Sequence similarities
Belongs to the constitutive coactivator of PPAR-gamma family. -
Post-translational
modificationsArg-982 is dimethylated, probably to asymmetric dimethylarginine.
Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure. -
Cellular localization
Cytoplasm. Cell membrane. Translocates to plasma membrane upon ultraviolet exposure. - Information by UniProt
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Database links
- Entrez Gene: 100398882 Common marmoset
- Entrez Gene: 23196 Human
- Entrez Gene: 218236 Mouse
- Entrez Gene: 291019 Rat
- GenBank: NP_055427.2 Human
- Omim: 612265 Human
- SwissProt: Q9NZB2 Human
- SwissProt: Q6A0A9 Mouse
see all -
Alternative names
- C9orf10 antibody
- Chromosome 9 open reading frame 10 antibody
- Constitutive coactivator of PPAR gamma like protein 1 antibody
see all
Images
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Western blot - Anti-FAM120A/OSSA antibody (ab156695)All lanes : Anti-FAM120A/OSSA antibody (ab156695) at 1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Predicted band size: 121 kDa
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Immunoprecipitation - Anti-FAM120A/OSSA antibody (ab156695)Detection of FAM120A/OSSA by Western blot of immunoprecipitate.
Immunoprecipitation of FAM120A/OSSA (6 µg of antibody per mg of Jurkat whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: alternative rabbit anti-FAM120A/OSSA antibody
Lane 2: ab156695
Lane 3: Control IgG
For the subsequent Western blot of immunoprecipitates, ab156695 was used at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
This product has been referenced in:
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). Read more (PubMed: 30247708) »
