Overview

  • Product name

    Anti-FAM120A/OSSA antibody
    See all FAM120A/OSSA primary antibodies
  • Description

    Rabbit polyclonal to FAM120A/OSSA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human FAM120A/OSSA (internal sequence). The exact sequence is proprietary.
    Database link: Q9NZB2

  • Positive control

    • ICC/IF: A431 cells. IHC-P: Human breast cancer tissue. WB: MCF7 whole cell lysate.
  • General notes

     This product was previously labelled as FAM120A

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 122 kDa.

Target

  • Function

    May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.
  • Tissue specificity

    Ubiquitously expressed. Highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa (at protein level).
  • Sequence similarities

    Belongs to the constitutive coactivator of PPAR-gamma family.
  • Post-translational
    modifications

    Arg-982 is dimethylated, probably to asymmetric dimethylarginine.
    Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure.
  • Cellular localization

    Cytoplasm. Cell membrane. Translocates to plasma membrane upon ultraviolet exposure.
  • Information by UniProt
  • Database links

  • Alternative names

    • C9orf10 antibody
    • Chromosome 9 open reading frame 10 antibody
    • Constitutive coactivator of PPAR gamma like protein 1 antibody
    • Constitutive coactivator of PPAR-gamma-like protein 1 antibody
    • DNA polymerase transactivated protein 5 antibody
    • DNA polymerase transactivated protein 1 antibody
    • DNAPTP1 antibody
    • DNAPTP5 antibody
    • F120A_HUMAN antibody
    • FAM120A antibody
    • Family with sequence similarity 120A antibody
    • Hypothetical protein LOC23196 antibody
    • KIAA0183 antibody
    • LOC23196 antibody
    • MGC111527 antibody
    • MGC133257 antibody
    • OSSA antibody
    • Oxidative stress-associated Src activator antibody
    • Protein FAM120A antibody
    see all

Images

  • Anti-FAM120A/OSSA antibody (ab229254) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 122 kDa



    7.5% SDS-PAGE

  • A431 (human epidermoid carcinoma cell line) cells stained for FAM120A/OSSA (green) using ab229254 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded human breast cancer tissue stained for FAM120A/OSSA with ab229254 at 1/250 dilution in immunohistochemical analysis.

References

ab229254 has not yet been referenced specifically in any publications.

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