Anti-FAM126A antibody (ab241202)
Key features and details
- Rabbit polyclonal to FAM126A
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAM126A antibody
See all FAM126A primary antibodies -
Description
Rabbit polyclonal to FAM126A -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human FAM126A aa 400-450. The exact sequence is proprietary.
Database link: Q9BYI3 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
ab241202 has not performed satisfactorily when used for WB of FAM126A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of FAM126A.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to FAM126A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. 8 - 15 µl/mg lysate |
Target
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Function
Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane (PubMed:26571211). The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis (PubMed:26571211). FAM126A plays a key role in oligodendrocytes formation, a cell type with expanded plasma membrane that requires generation of PtdIns(4)P (PubMed:26571211). Its role in oligodendrocytes formation probably explains its importance in myelination of the central and peripheral nervous system (PubMed:26571211, PubMed:16951682). May also have a role in the beta-catenin/Lef signaling pathway. -
Tissue specificity
Widely expressed. Highest levels in heart, brain, placenta, spleen and testis. -
Involvement in disease
Leukodystrophy, hypomyelinating, 5 -
Sequence similarities
Belongs to the FAM126 family. -
Cellular localization
Cytoplasm, cytosol. Cell membrane. Localizes to the cytosol and is recruited to the plasma membrane following interaction with other components of the phosphatidylinositol 4-kinase (PI4K) complex. - Information by UniProt
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Database links
- Entrez Gene: 84668 Human
- Entrez Gene: 84652 Mouse
- Omim: 610531 Human
- SwissProt: Q9BYI3 Human
- SwissProt: Q6P9N1 Mouse
- Unigene: 85603 Human
- Unigene: 304976 Mouse
- Unigene: 486278 Mouse
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Alternative names
- Down regulated by Ctnnb1 a antibody
- Down regulated by CTNNB1 protein A antibody
- Down-regulated by CTNNB1 protein A antibody
see all
Images
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Immunoprecipitation - Anti-FAM126A antibody (ab241202)
FAN126A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241202 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab241202 at 1/400 dilution.
Lane 1: ab241202 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241202 has not yet been referenced specifically in any publications.
