Key features and details
- Rabbit polyclonal to FAM126A
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM126A antibody
See all FAM126A primary antibodies
DescriptionRabbit polyclonal to FAM126A
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human FAM126A aa 400-450. The exact sequence is proprietary.
Database link: Q9BYI3
- IP: HeLa whole cell lysate.
ab241202 has not performed satisfactorily when used for WB of FAM126A in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of FAM126A.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to FAM126A immobilized on solid support.
Our Abpromise guarantee covers the use of ab241202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
8 - 15 µl/mg lysate
FunctionComponent of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane (PubMed:26571211). The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis (PubMed:26571211). FAM126A plays a key role in oligodendrocytes formation, a cell type with expanded plasma membrane that requires generation of PtdIns(4)P (PubMed:26571211). Its role in oligodendrocytes formation probably explains its importance in myelination of the central and peripheral nervous system (PubMed:26571211, PubMed:16951682). May also have a role in the beta-catenin/Lef signaling pathway.
Tissue specificityWidely expressed. Highest levels in heart, brain, placenta, spleen and testis.
Involvement in diseaseLeukodystrophy, hypomyelinating, 5
Sequence similaritiesBelongs to the FAM126 family.
Cellular localizationCytoplasm, cytosol. Cell membrane. Localizes to the cytosol and is recruited to the plasma membrane following interaction with other components of the phosphatidylinositol 4-kinase (PI4K) complex.
- Information by UniProt
- Down regulated by Ctnnb1 a antibody
- Down regulated by CTNNB1 protein A antibody
- Down-regulated by CTNNB1 protein A antibody
FAN126A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241202 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab241202 at 1/400 dilution.
Lane 1: ab241202 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241202 has not yet been referenced specifically in any publications.