Recombinant Anti-FAM134B antibody [EPR10608(B)] - BSA and Azide free (ab240125)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10608(B)] to FAM134B - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-FAM134B antibody [EPR10608(B)] - BSA and Azide free
See all FAM134B primary antibodies -
Description
Rabbit monoclonal [EPR10608(B)] to FAM134B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human FAM134B. The exact sequence is proprietary.
-
General notes
Ab240125 is the carrier-free version of ab151755. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240125 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10608(B) -
Isotype
IgG
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab240125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use at an assay dependent concentration. Predicted molecular weight: 54 kDa. |
Target
-
Function
Required for long-term survival of nociceptive and autonomic ganglion neurons. -
Involvement in disease
Defects in FAM134B are the cause of hereditary sensory and autonomic neuropathy type 2B (HSAN2B) [MIM:613115]. A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN2B is an autosomal recessive disorder characterized by impairment of pain, temperature and touch sensation. Onset occurs in the first or second decade, with impaired nociception and progressive mutilating ulceration of the hands and feet with osteomyelitis and acroosteolysis. Amputations of the hands and feet are common. Autonomic dysfunction includes hyperhidrosis, urinary incontinence, and slow pupillary light response. -
Sequence similarities
Belongs to the FAM134 family. -
Cellular localization
Golgi apparatus > cis-Golgi network membrane. - Information by UniProt
-
Database links
- Entrez Gene: 54463 Human
- Entrez Gene: 66270 Mouse
- Entrez Gene: 619558 Rat
- Omim: 613114 Human
- SwissProt: Q9H6L5 Human
- SwissProt: Q8VE91 Mouse
- SwissProt: Q5FVM3 Rat
- Unigene: 720770 Human
see all -
Alternative names
- F134B_HUMAN antibody
- FAM134B antibody
- family with sequence similarity 134 member B antibody
see all
Images
-
All lanes : Anti-FAM134B antibody [EPR10608(B)] (ab151755) at 1/1000 dilution
Lane 1 : Fetal brain cell lysate
Lane 2 : Human cerebellum cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDaThis data was developed using ab151755, the same antibody clone in a different buffer formulation.
-
All lanes : Anti-FAM134B antibody [EPR10608(B)] (ab151755) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysates
Lane 2 : Rat brain tissue lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 minutesThis data was developed using ab151755, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab240125 has not yet been referenced specifically in any publications.