Overview

  • Product name

    Anti-FAM134C antibody - C-terminal
    See all FAM134C primary antibodies
  • Description

    Rabbit polyclonal to FAM134C - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human FAM134C aa 416-466 (C terminal). The exact sequence is proprietary. NP_835227.1
    Database link: Q86VR2

  • Positive control

    • HeLa, 293T, and Jurkat whole cell lysate (ab7899).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-FAM134C antibody - C-terminal (ab202125) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa


    Exposure time: 30 seconds
  • Lane 1: Detection of FAM134C in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 0.5 or 1.0 mg per IP reaction, 20% of IP loaded) using ab202125 at 6 µg per reaction, and at 1 µg/ml for subsequent Western blot detection.
    Lane 2: controle IgG
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab202125 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa/HEK293T)
Gel Running Conditions
Reduced Denaturing (8-15%)
Loading amount
50 µg
Specification
HeLa/HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 29 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 22 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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