Key features and details
- Rabbit polyclonal to FAM149B1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the FAM149 family.
- Information by UniProt
- F149B_HUMAN antibody
- FAM149B1 antibody
- Family with sequence similarity 149 member B1 antibody
Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoskeleton (microtubules). Recommended concentration of ab122541 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122541, at 1/200 dilution, staining FAM149B1 in paraffin-embedded Human bronchus tissue by Immunohistochemistry.
ab122541 has not yet been referenced specifically in any publications.