Overview

  • Product name

  • Description

    Rabbit polyclonal to FAM149B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human FAM149B1 aa 20-70 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    TKHALNHHPPPEKLEEISPTSDSHEKDTSSQSKSDITRESSFTSADTGNS L


    Database link: Q96BN6

  • Positive control

    • IHC-P: Human kidney tissue. WB: Mouse bone lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 65 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded human spleen tissue labeling FAM149B1 using ab214177 at 1/200, followed by secondary detection and DAB staining.

  • Anti-FAM149B1 antibody (ab214177) at 1/300 dilution + mouse bone lysate

    Secondary
    Goat anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution

    Predicted band size: 65 kDa

References

ab214177 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab214177.
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