Key features and details
- Rabbit polyclonal to FAM154A/SAXO1 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM154A/SAXO1 antibody - C-terminal
DescriptionRabbit polyclonal to FAM154A/SAXO1 - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
- WB: FAM154A/SAXO1 overexpression HEK-293T lysate. IHC-P: Human testis tissue.
This product was previously labelled as FAM154A
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 55 kDa.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in the regulation of cilium length. Stabilizes microtubules at low temperature.
Tissue specificityWidely expressed, with highest levels in testis. Expressed in mature spermatozoa (at protein level).
Sequence similaritiesBelongs to the FAM154 family.
Developmental stageIn retinal pigment epithelium (RPE1) cell line, after 24 hours of serum starvation to stimulate primary cilium production, present at the mother and daughter basal bodies. At early time points of ciliogenesis, not detected in the axoneme. At later time points, expressed along the axoneme, with increasing levels as maturation of the cilium proceeds.
DomainThe Mn regions are involved in microtubule-binding and stabilization at low temperature. They are required and sufficient for cilium targeting.
The N-terminal region (residues 1-29) might play a role in centriole retention.
Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, flagellum axoneme. In multi-ciliated cells, localizes to the basal bodies and in non-ciliated cells, to the centrosome. In spermatozoa, colocalizes with microtubules along the length of the axoneme from its proximal end to its distal tip and with tubulin at the distal end of the flagellum and at the proximal centriole.
- Information by UniProt
- 4930500O09Rik antibody
- C9orf138 antibody
- Family with sequence similarity 154 member A antibody
Paraffin-embedded human testis tissue stained for FAM154A/SAXO1 using ab224455 at 1/20 dilution in immunohistochemical analysis.
All lanes : Anti-FAM154A/SAXO1 antibody - C-terminal (ab224455) at 1/100 dilution
Lane 1 : Negative control (vector only transfected HEK-293T cell lysate)
Lane 2 : FAM154A/SAXO1 overexpression HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) co-expressed with a C-terminal myc-DDK tag, cell lysate.
Developed using the ECL technique.
Predicted band size: 55 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224455 has not yet been referenced specifically in any publications.