Key features and details
- Rabbit polyclonal to FAM171A1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM171A1 antibody
DescriptionRabbit polyclonal to FAM171A1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment within Human FAM171A1 (C terminal). The exact sequence is proprietary.
Database link: Q5VUB5
- WB: HepG2 and HCT 116 whole cell lysates. IHC-P: Human hepatoma tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 98 kDa.|
RelevanceThe exact function of FAM171A1, which is also known as C10orf38, remains unknown.
Cellular localizationMembrane; Single-pass type I membrane protein (Potential)
- Astroprincin antibody
- C10orf38 antibody
- chromosome 10 open reading frame 38 antibody
All lanes : Anti-FAM171A1 antibody (ab229247) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 98 kDa
7.5% SDS-PAGE gel.
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FAM171A1 (green) using ab229247 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Paraffin-embedded human hepatoma tissue stained for FAM171A1 using ab229247 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229247 has not yet been referenced specifically in any publications.