Product nameAnti-FAM172A antibody
DescriptionRabbit polyclonal to FAM172A
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
antigen, corresponding to amino acids 19-135 of Human FAM172A (Uniprot: Q8WUF8)
- Human Cerebellum tissue, RT-4 lysate, U-251 MG lysate, Liver lysate and Tonsil lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesBelongs to the UPF0528 family.
- Information by UniProt
- C5orf21 antibody
- F172A_HUMAN antibody
- fam172a antibody
All lanes : Anti-FAM172A antibody (ab121364) at 0.4 µg/ml
Lane 1 : NIH-3T3 cell lysate
Lane 2 : NBT-II cell lysate
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121364 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121364, at 1/200 dilution, staining FAM172A in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
This product has been referenced in:
- Liu W et al. Expression of family with sequence similarity 172 member A and nucleotide-binding protein 1 is associated with the poor prognosis of colorectal carcinoma. Oncol Lett 14:3587-3593 (2017). Read more (PubMed: 28927116) »
- Liu W et al. miR-27a promotes proliferation, migration, and invasion of colorectal cancer by targeting FAM172A and acts as a diagnostic and prognostic biomarker. Oncol Rep 37:3554-3564 (2017). Read more (PubMed: 28440497) »