Overview

  • Product name

    Anti-FAM172A antibody
  • Description

    Rabbit polyclonal to FAM172A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen, corresponding to amino acids 19-135 of Human FAM172A (Uniprot: Q8WUF8)

  • Positive control

    • Human Cerebellum tissue, RT-4 lysate, U-251 MG lysate, Liver lysate and Tonsil lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • All lanes : Anti-FAM172A antibody (ab121364) at 0.4 µg/ml

    Lane 1 : NIH-3T3 cell lysate
    Lane 2 : NBT-II cell lysate
  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121364 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121364, at 1/200 dilution, staining FAM172A in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Liu W  et al. Expression of family with sequence similarity 172 member A and nucleotide-binding protein 1 is associated with the poor prognosis of colorectal carcinoma. Oncol Lett 14:3587-3593 (2017). Read more (PubMed: 28927116) »
  • Liu W  et al. miR-27a promotes proliferation, migration, and invasion of colorectal cancer by targeting FAM172A and acts as a diagnostic and prognostic biomarker. Oncol Rep 37:3554-3564 (2017). Read more (PubMed: 28440497) »
See all 2 Publications for this product

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