Key features and details
- Rabbit polyclonal to FAM174A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM174A antibody
DescriptionRabbit polyclonal to FAM174A
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human hippocampus tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the FAM174 family.
- Information by UniProt
- F174A_HUMAN antibody
- FAM174A antibody
- HCV NS5A-transactivated protein 6 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus. Recommended concentration of ab121607 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121607 at 1/120 dilution staining FAM174A in Paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity with a granular pattern in neuronal cells.
All lanes : Anti-FAM174A antibody (ab121607) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate [Co-expressed with a C-terminal proprietary tag (~3.1 kDa)] in mammalian HEK293T cells.
Developed using the ECL technique.
ab121607 has not yet been referenced specifically in any publications.