Key features and details
- Mouse polyclonal to FAM175B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM175B antibody
See all FAM175B primary antibodies
DescriptionMouse polyclonal to FAM175B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant protein, corresponding to amino acids 105-415a.a. of Human FAM175B
- FAM175B transfected 293T cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 47 kDa).|
RelevanceFAM175B (ABRO1) is a 415 amino acid protein, sharing 39% identity to ABRA1 in the N terminal two thirds of the protein (amino acids 1 to 260), including a coiled-coil domain and a region called the ABR domain. However, ABRO1 lacks the phosphorylated ser-X-X-phe motif of ABRA1 and, unlike ABRA1, does not bind to BRCA1.
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All lanes : Anti-FAM175B antibody (ab68801) at 1/500 dilution
Lane 1 : FAM175B (1-311 a.a.: 34.7kDa) transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L) at 1/5000 dilution
Predicted band size: 47 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
ab68801 has been referenced in 1 publication.
- Zhao Y et al. FAM175B promotes apoptosis by inhibiting ATF4 ubiquitination in esophageal squamous cell carcinoma. Mol Oncol 13:1150-1165 (2019). PubMed: 30854784