Overview

  • Product name

  • Description

    Mouse polyclonal to FAM175B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant protein, corresponding to amino acids 105-415a.a. of Human FAM175B

  • Positive control

    • FAM175B transfected 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 47 kDa).

Target

  • Relevance

    FAM175B (ABRO1) is a 415 amino acid protein, sharing 39% identity to ABRA1 in the N terminal two thirds of the protein (amino acids 1 to 260), including a coiled-coil domain and a region called the ABR domain. However, ABRO1 lacks the phosphorylated ser-X-X-phe motif of ABRA1 and, unlike ABRA1, does not bind to BRCA1.
  • Database links

  • Alternative names

    • AA589499 antibody
    • Abraxas brother 1 antibody
    • ABRO 1 antibody
    • ABRO antibody
    • ABRO1 antibody
    • AI853413 antibody
    • C430003P19Rik antibody
    • Em:AC068896.4 antibody
    • Family with sequence similarity 175, member B antibody
    • FLJ22338 antibody
    • hypothetical protein LOC23172 antibody
    • KIAA0157 antibody
    • OTTHUMP00000020697 antibody
    • RGD1308918 antibody
    see all

Images

  • All lanes : Anti-FAM175B antibody (ab68801) at 1/500 dilution

    Lane 1 : FAM175B (1-311 a.a.: 34.7kDa) transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L) at 1/5000 dilution

    Predicted band size: 47 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Zhao Y  et al. FAM175B promotes apoptosis by inhibiting ATF4 ubiquitination in esophageal squamous cell carcinoma. Mol Oncol 13:1150-1165 (2019). Read more (PubMed: 30854784) »
See 1 Publication for this product

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