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Rabbit polyclonal to FAM179A
internal sequence amino acids 742-834 (GLSCNGPRLV GLRSTLQGRG EMVEQLRELT RLLEAKDFRS RMEGVGQLLE LCKAKTELVT AHLVQVFDAF TPRLQDSNKK VNQWALESFA KMI) of the Human FAM179A protein (Q6ZUX3).
- IHC-P: Human colon tissue
WB: RT4, U251 MG, Human Liver and Tonsil cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Belongs to the FAM179 family.
Information by UniProt
- F179A_HUMAN antibody
- FAM179A antibody
- Family with sequence similarity 179 member A antibody
- Protein FAM179A antibody
Immunocytochemistry/ Immunofluorescence - Anti-FAM179A antibody (ab122395)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus and nucleoli. Recommended concentration of ab122395 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-FAM179A antibody (ab122395)
All lanes : Anti-FAM179A antibody (ab122395) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma cell lysate
Lane 4 : Liver cell lysate
Lane 5 : Tonsil cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM179A antibody (ab122395)
ab122395, at 1/50, staining FAM179A in paraffin embedded Human colon tissue by immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"