Product nameAnti-FAM181A antibody
DescriptionRabbit polyclonal to FAM181A
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
antigen, corresponding to C-terminus amino acids 232-352 of the Human FAM181A protein (Q8N9Y4).
- Human testis tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/250.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the FAM181 family.
- Information by UniProt
- C14orf152 antibody
- F181A_HUMAN antibody
- FAM181A antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli. Recommended concentration of ab121144 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lanes 2-3 : Anti-FAM181A antibody (ab121144)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing FAM181A with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemical staining of Human testis with ab121144 at 1/100 dilution
ab121144 has not yet been referenced specifically in any publications.