Key features and details
- Rabbit polyclonal to FAM18B - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM18B antibody - C-terminal
DescriptionRabbit polyclonal to FAM18B - C-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: FAM18B overexpression HEK-293T lysate. IHC-P: Human lung tissue. ICC/IF: U-251 MG cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/250. Predicted molecular weight: 23 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Sequence similaritiesBelongs to the TVP23 family.
- Information by UniProt
- CGI 148 antibody
- FAM18B1 antibody
- Family with sequence similarity 18 member B1 antibody
All lanes : Anti-FAM18B antibody - C-terminal (ab224418) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : FAM18B overexpression HEK-293T lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Predicted band size: 23 kDa
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for FAM18B (green) using ab224418 at 4 µg/ml in ICC/IF.
Paraffin-embedded human lung tissue stained for FAM18B using ab224418 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224418 has not yet been referenced specifically in any publications.