Key features and details
- Rabbit polyclonal to FAM198B
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM198B antibody
DescriptionRabbit polyclonal to FAM198B
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- WB: FAM198B over-expression - HEK-293T whole cell lysate; IHC-P: Human adrenal gland tissue; ICC: HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 58 kDa.|
Sequence similaritiesBelongs to the FAM198 family.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- AD021 antibody
- AD036 antibody
- C4orf18 antibody
All lanes : Anti-FAM198B antibody (ab224234) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : FAM198B over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 58 kDa
Immunohistochemical analysis of human adrenal glandctissue labeling FAM198B in the cytoplasm of glandular cells with ab224234 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of HepG2 (human liver hepatocellular carcinoma cell line) whole cells labeling FAM198B (green) in nucleus, nucleoli fibrillar center and the Golgi apparatus with ab224234 at 2 µg/ml.
ab224234 has not yet been referenced specifically in any publications.