Key features and details
- Rabbit polyclonal to FAM199X
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM199X antibody
DescriptionRabbit polyclonal to FAM199X
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to amino acids 181-309 of Human FAM199X.
- Human cerebral cortex tissue; RT4 lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
Sequence similaritiesBelongs to the FAM199 family.
- Information by UniProt
- CXorf39 antibody
- F199X_HUMAN antibody
- fam199x antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab121167 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121167 at 1/10 dilution staining FAM199X in Paraffin-emebedded Human cerebral cortex tissue by Immunohistochemistry.
Note: strong nuclear and moderate cytoplasmic positivity in neuronal cells.
All lanes : Anti-FAM199X antibody (ab121167) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MG lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab121167 has not yet been referenced specifically in any publications.