Overview

  • Product name
  • Description
    Rabbit polyclonal to FAM20C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Monkey
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 233-512 of Human FAM20C (UniProt ID: Q8IXL6).

  • Positive control
    • Molt4 whole cell lysate; Hep3B cells; Human MDA-MB468 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab154740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Calcium-binding protein which may play a role in dentin mineralization.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Defects in FAM20C are the cause of Raine syndrome (RNS) [MIM:259775]. RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly.
  • Sequence similarities
    Belongs to the FAM20 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C76981 antibody
    • Dentin matrix protein 4 antibody
    • DKFZp547C074 antibody
    • DMP-4 antibody
    • DMP4 antibody
    • DMP4_HUMAN antibody
    • Extracellular serine/threonine protein kinase Fam20C antibody
    • Fam20c antibody
    • Family with sequence similarity 20 member C antibody
    • GEF CK antibody
    • Golgi enriched fraction casein kinase antibody
    • Protein FAM20C antibody
    • RNS antibody
    see all

Images

  • Anti-FAM20C antibody (ab154740) at 1/500 dilution + Molt4 whole cell lysate at 30 µg

    Predicted band size: 66 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed Hep3B cells labeling FAM20C with ab154740 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft labeling FAM20C with ab154740 at 1/100 dilution.

References

This product has been referenced in:
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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