Anti-FAM20C antibody (ab154740)
Key features and details
- Rabbit polyclonal to FAM20C
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FAM20C antibody
See all FAM20C primary antibodies -
Description
Rabbit polyclonal to FAM20C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Monkey -
Immunogen
Recombinant fragment corresponding to a region within amino acids 233-512 of Human FAM20C (UniProt ID: Q8IXL6).
-
Positive control
- Molt4 whole cell lysate; Hep3B cells; Human MDA-MB468 xenograft.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 66 kDa.
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 66 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Calcium-binding protein which may play a role in dentin mineralization. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in FAM20C are the cause of Raine syndrome (RNS) [MIM:259775]. RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly. -
Sequence similarities
Belongs to the FAM20 family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 56975 Human
- Entrez Gene: 80752 Mouse
- Entrez Gene: 304334 Rat
- Omim: 611061 Human
- SwissProt: Q8IXL6 Human
- SwissProt: Q5MJS3 Mouse
- Unigene: 134742 Human
- Unigene: 320355 Mouse
-
Alternative names
- C76981 antibody
- Dentin matrix protein 4 antibody
- DKFZp547C074 antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft labeling FAM20C with ab154740 at 1/100 dilution.
-
Anti-FAM20C antibody (ab154740) at 1/500 dilution + Molt4 whole cell lysate at 30 µg
Predicted band size: 66 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed Hep3B cells labeling FAM20C with ab154740 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab154740 has been referenced in 6 publications.
- Feng J et al. Fam20C Overexpression Predicts Poor Outcomes and is a Diagnostic Biomarker in Lower-Grade Glioma. Front Genet 12:757014 (2021). PubMed: 34970298
- Du S et al. Secretory Pathway Kinase FAM20C, a Marker for Glioma Invasion and Malignancy, Predicts Poor Prognosis of Glioma. Onco Targets Ther 13:11755-11768 (2020). PubMed: 33239887
- Hernández-Zavala A et al. Two Novel FAM20C Variants in A Family with Raine Syndrome. Genes (Basel) 11:N/A (2020). PubMed: 32093234
- Cozza G et al. The Golgi 'casein kinase' Fam20C is a genuine 'phosvitin kinase' and phosphorylates polyserine stretches devoid of the canonical consensus. FEBS J 285:4674-4683 (2018). PubMed: 30387551
- Cozza G et al. Fam20C is under the control of sphingolipid signaling in human cell lines. FEBS J 284:1246-1257 (2017). PubMed: 28236661
- Knab VM et al. Acute Parathyroid Hormone Injection Increases C-Terminal but Not Intact Fibroblast Growth Factor 23 Levels. Endocrinology 158:1130-1139 (2017). PubMed: 28324013