• Product name
  • Description
    Mouse polyclonal to FAM21C
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human FAM21C protein (AAH06456, 1 a.a. ~ 283 a.a)

  • Positive control
    • FAM21C transfected 293T cell lysate. IHC-P: FFPE human lung tissue sections.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituent: Whole serum
  • Purity
    Whole antiserum
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab72720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
IP Use at an assay dependent concentration. PubMed: 20923837
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes. Plays a role in fluid-phase endocytosis, a process exploited by vaccinia intracellular mature virus (IMV) to enter cells. As a result, may facilitate the penetration of IMV into cells.
  • Sequence similarities
    Belongs to the FAM21 family.
  • Cellular localization
    Early endosome membrane. Cell membrane. Partially colocalizes with RAB11A, a recycling endosome marker. Associates with lipid raft microdomains on the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FA21C_HUMAN antibody
    • FAM21A antibody
    • FAM21C antibody
    • Family with sequence similarity 21 member C antibody
    • Hypothetical protein LOC253725 antibody
    • KIAA0592 antibody
    • Protein FAM21C antibody
    • Vaccinia virus penetration factor antibody
    • VPEF antibody
    • WASH complex subunit FAM21C antibody
    see all


  • IHC image of FAM21C staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72720, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-FAM21C antibody (ab72720) at 1/500 dilution

    Lane 1 : FAM21C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 31 kDa
    Observed band size: 35 kDa (why is the actual band size different from the predicted?)

  • ab72720 used in Immunoprecipitation.


This product has been referenced in:
  • Harbour ME  et al. The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics. J Cell Sci 123:3703-17 (2010). IP ; Human . Read more (PubMed: 20923837) »

See 1 Publication for this product

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