Anti-FAM29A antibody (ab150806)
Key features and details
- Rabbit polyclonal to FAM29A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAM29A antibody
See all FAM29A primary antibodies -
Description
Rabbit polyclonal to FAM29A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 729-831 of Human FAM29A (UniProt: Q7Z4H7-1).
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Positive control
- Human urinary bladder tissue; RT4 and U 251 MG lysates; U 251 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
1/100 - 1/250. Predicted molecular weight: 109 kDa.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
1/100 - 1/250. Predicted molecular weight: 109 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
FAM29A is required for progression through mitosis. It promotes the nucleation of microtubules from the spindle through recruitment of NEDD1 and gamma tubulin. -
Cellular localization
Cytoplasm, Cytoskeleton, Microtubule -
Database links
- Entrez Gene: 54801 Human
- Omim: 613433 Human
- SwissProt: Q7Z4H7 Human
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Alternative names
- Dgt6 antibody
- Dim gamma tubulin homolog antibody
- Dim gamma-tubulin 6, Drosophila, homolog of antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling FAM29A with ab150806 antibody at a dilution of 1/200.
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All lanes : Anti-FAM29A antibody (ab150806) at 1/100 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 109 kDa -
Immunofluorescent analysis of U 251 MG cells labeling FAM29A with ab150806 antibody at a dilution of 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab150806 has not yet been referenced specifically in any publications.