Overview

  • Product name

  • Description

    Rabbit polyclonal to FAM29A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 729-831 of Human FAM29A (UniProt: Q7Z4H7-1).

  • Positive control

    • Human urinary bladder tissue; RT4 and U 251 MG lysates; U 251 MG cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/100 - 1/250. Predicted molecular weight: 109 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    FAM29A is required for progression through mitosis. It promotes the nucleation of microtubules from the spindle through recruitment of NEDD1 and gamma tubulin.
  • Cellular localization

    Cytoplasm, Cytoskeleton, Microtubule
  • Database links

  • Alternative names

    • Dgt6 antibody
    • Dim gamma tubulin homolog antibody
    • Dim gamma-tubulin 6, Drosophila, homolog of antibody
    • Family with sequence similarity 29 member A antibody
    • FLJ20060 antibody
    • HAUS augmin like complex subunit 6 antibody
    • HAUS6 antibody
    • KIAA15741 antibody
    • RP11-296P7.3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling FAM29A with ab150806 antibody at a dilution of 1/200.
  • All lanes : Anti-FAM29A antibody (ab150806) at 1/100 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Predicted band size: 109 kDa

  • Immunofluorescent analysis of U 251 MG cells labeling FAM29A with ab150806 antibody at a dilution of 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.

References

ab150806 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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