Key features and details
- Rabbit polyclonal to FAM29A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM29A antibody
See all FAM29A primary antibodies
DescriptionRabbit polyclonal to FAM29A
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human urinary bladder tissue; RT4 and U 251 MG lysates; U 251 MG cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||1/100 - 1/250. Predicted molecular weight: 109 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceFAM29A is required for progression through mitosis. It promotes the nucleation of microtubules from the spindle through recruitment of NEDD1 and gamma tubulin.
Cellular localizationCytoplasm, Cytoskeleton, Microtubule
- Dgt6 antibody
- Dim gamma tubulin homolog antibody
- Dim gamma-tubulin 6, Drosophila, homolog of antibody
Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling FAM29A with ab150806 antibody at a dilution of 1/200.
All lanes : Anti-FAM29A antibody (ab150806) at 1/100 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 109 kDa
Immunofluorescent analysis of U 251 MG cells labeling FAM29A with ab150806 antibody at a dilution of 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
ab150806 has not yet been referenced specifically in any publications.