Anti-FAM29A antibody (ab241284)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FAM29A antibody
See all FAM29A primary antibodies -
Description
Rabbit polyclonal to FAM29A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FAM29A aa 475-525. The exact sequence is proprietary.
Database link: Q7Z4H7 -
Positive control
- WB: HeLa, HEK-293T and Jurkat cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241284 was affinity purified using an epitope specific to FAM29A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2500. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Relevance
FAM29A is required for progression through mitosis. It promotes the nucleation of microtubules from the spindle through recruitment of NEDD1 and gamma tubulin. -
Cellular localization
Cytoplasm, Cytoskeleton, Microtubule -
Database links
- Entrez Gene: 54801 Human
- Omim: 613433 Human
- SwissProt: Q7Z4H7 Human
- Unigene: 533468 Human
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Alternative names
- Dgt6 antibody
- Dim gamma tubulin homolog antibody
- Dim gamma-tubulin 6, Drosophila, homolog of antibody
see all
Images
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Western blot - Anti-FAM29A antibody (ab241284)All lanes : Anti-FAM29A antibody (ab241284) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 10 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-FAM29A antibody (ab241284)FAM29A was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241284 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241284 at 1 µg/ml.
Lane 1: ab241284 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Datasheets and documents
References
ab241284 has not yet been referenced specifically in any publications.
