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    fam29a-antibody-ab241284.pdf

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Cell Biology Cell Cycle Cell differentiation
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Anti-FAM29A antibody (ab241284)

  • Datasheet
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Abpromise

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Western blot - Anti-FAM29A antibody (ab241284)
  • Immunoprecipitation - Anti-FAM29A antibody (ab241284)

Key features and details

  • Rabbit polyclonal to FAM29A
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-FAM29A antibody
    See all FAM29A primary antibodies
  • Description

    Rabbit polyclonal to FAM29A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FAM29A aa 475-525. The exact sequence is proprietary.
    Database link: Q7Z4H7

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab241284 was affinity purified using an epitope specific to FAM29A immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab241284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    FAM29A is required for progression through mitosis. It promotes the nucleation of microtubules from the spindle through recruitment of NEDD1 and gamma tubulin.
  • Cellular localization

    Cytoplasm, Cytoskeleton, Microtubule
  • Database links

    • Entrez Gene: 54801 Human
    • Omim: 613433 Human
    • SwissProt: Q7Z4H7 Human
    • Unigene: 533468 Human
    • Alternative names

      • Dgt6 antibody
      • Dim gamma tubulin homolog antibody
      • Dim gamma-tubulin 6, Drosophila, homolog of antibody
      • Family with sequence similarity 29 member A antibody
      • FLJ20060 antibody
      • HAUS augmin like complex subunit 6 antibody
      • HAUS6 antibody
      • KIAA15741 antibody
      • RP11-296P7.3 antibody
      see all

    Images

    • Western blot - Anti-FAM29A antibody (ab241284)
      Western blot - Anti-FAM29A antibody (ab241284)
      All lanes : Anti-FAM29A antibody (ab241284) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 15 µg per lane.

      Developed using the ECL technique.

      Exposure time: 10 seconds


      Prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-FAM29A antibody (ab241284)
      Immunoprecipitation - Anti-FAM29A antibody (ab241284)

      FAM29A was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241284 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241284 at 1 µg/ml.

      Lane 1: ab241284 IP in Jurkat whole cell lysate.
      Lane 2: Control IgG IP in Jurkat whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab241284? Please let us know so that we can cite the reference in this datasheet.

    ab241284 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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