Anti-FAM29A antibody (ab241284)
Key features and details
- Rabbit polyclonal to FAM29A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAM29A antibody
See all FAM29A primary antibodies -
Description
Rabbit polyclonal to FAM29A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FAM29A aa 475-525. The exact sequence is proprietary.
Database link: Q7Z4H7 -
Positive control
- WB: HeLa, HEK-293T and Jurkat cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241284 was affinity purified using an epitope specific to FAM29A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2500. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Relevance
FAM29A is required for progression through mitosis. It promotes the nucleation of microtubules from the spindle through recruitment of NEDD1 and gamma tubulin. -
Cellular localization
Cytoplasm, Cytoskeleton, Microtubule -
Database links
- Entrez Gene: 54801 Human
- Omim: 613433 Human
- SwissProt: Q7Z4H7 Human
- Unigene: 533468 Human
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Alternative names
- Dgt6 antibody
- Dim gamma tubulin homolog antibody
- Dim gamma-tubulin 6, Drosophila, homolog of antibody
see all
Images
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Western blot - Anti-FAM29A antibody (ab241284)All lanes : Anti-FAM29A antibody (ab241284) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 10 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-FAM29A antibody (ab241284)FAM29A was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241284 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241284 at 1 µg/ml.
Lane 1: ab241284 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241284 has not yet been referenced specifically in any publications.
