Key features and details
- Rabbit polyclonal to FAM29A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM29A antibody
See all FAM29A primary antibodies
DescriptionRabbit polyclonal to FAM29A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human FAM29A aa 475-525. The exact sequence is proprietary.
Database link: Q7Z4H7
- WB: HeLa, HEK-293T and Jurkat cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241284 was affinity purified using an epitope specific to FAM29A immobilized on solid support.
Our Abpromise guarantee covers the use of ab241284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceFAM29A is required for progression through mitosis. It promotes the nucleation of microtubules from the spindle through recruitment of NEDD1 and gamma tubulin.
Cellular localizationCytoplasm, Cytoskeleton, Microtubule
- Dgt6 antibody
- Dim gamma tubulin homolog antibody
- Dim gamma-tubulin 6, Drosophila, homolog of antibody
All lanes : Anti-FAM29A antibody (ab241284) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
Prepared using NETN lysis buffer.
FAM29A was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241284 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241284 at 1 µg/ml.
Lane 1: ab241284 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241284 has not yet been referenced specifically in any publications.