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Our Abpromise guarantee covers the use of ab185298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500. Predicted molecular weight: 13 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling FAM32A with ab185298 at 1/200 dilution.
Immunofluorescent analysis of paraformaldehyde-fixed, Triton X-100-permeabilized A431 cells labeling FAM32A with ab185298 at 4 µg/ml.
ab185298 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"