Anti-FAM38A/PIEZO1 antibody (ab128245)

Reviews (5)Q&A (4)References (6)

Overview

  • Product name

    Anti-FAM38A/PIEZO1 antibody

  • Description

    Rabbit polyclonal to FAM38A/PIEZO1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISA, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit

  • Immunogen

    Synthetic peptide corresponding to Human FAM38A/PIEZO1 aa 2100-2200 (C terminal).
    (Peptide available as ab133015)

  • General notes

     This product was previously labelled as FAM38A

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.

  • Storage buffer

    Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 287 kDa.Can be blocked with FAM38A/PIEZO1 peptide (ab133015).
ELISA Use at an assay dependent concentration.
IHC-Fr 1/50 - 1/500.
IHC-P Use at an assay dependent concentration. PubMed: 25385580

Target

  • Function

    Component of mechanosensitive channel required for the mechanosensitive currents. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling.

  • Tissue specificity

    Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes.

  • Sequence similarities

    Belongs to the PIEZO family.

  • Cellular localization

    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DHS antibody
    • Fam38a antibody
    • Family with sequence similarity 38 member A antibody
    • KIAA0233 antibody
    • Membrane protein induced by beta-amyloid treatment antibody
    • Mib antibody
    • PIEZ1_HUMAN antibody
    • Piezo-type mechanosensitive ion channel component 1 antibody
    • PIEZO1 antibody
    • Protein FAM38A antibody
    • Protein FAM38B antibody
    • Protein PIEZO1 antibody
    see all

References

This product has been referenced in:

  • Shi R  et al. Long noncoding antisense RNA FAM83A-AS1 promotes lung cancer cell progression by increasing FAM83A. J Cell Biochem N/A:N/A (2019). Read more (PubMed: 30659636) »
  • Wang YY  et al. Piezo1 mediates neuron oxygen-glucose deprivation/reoxygenation injury via Ca2+/calpain signaling. Biochem Biophys Res Commun 513:147-153 (2019). Read more (PubMed: 30948157) »
See all 6 Publications for this product

Publishing research using ab128245? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-9 of 9 Abreviews or Q&A

Question

1) Frozen sections. We either do immersed PFA fixation prior to freezing samples for sectioning, or we do fresh frozen tissue and fix with PFA prior to immunohistochemistry.

2) Mouse is the priority, but it would be beneficial if it also worked in rat and human.

Read More
Answer

Thanks for the additional details.

Since you're using frozen sections, I'd suggest using ab128245 as its already been tested in IHC-Fr on human samples. Although it has not been tested on mouse or rat samples that we know of, based on the similarity of theimmunogen sequenceto mouse andrat proteinab128245 is predicted to react with these species. Since you are interested in testing this antibody in untested species, you may be interested in the testing discount program described below where we describe how you can earn a FREE primary antibody of your choice:

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To our knowledge, ab128245 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab128245 now, test it inmouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab128245 in mouse. I will then send a discount code. This code must be issued before purchasingab 128245 so please wait for my reply before ordering.

2. Purchase ab128245 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab128245 turns out to be unsuitable for mouse you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Immunocytochemistry/ Immunofluorescence abreview for Anti-FAM38A/PIEZO1 antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (primary neuron culture)
Permeabilization
Yes - 0.05% Triton-X in PBS
Specification
primary neuron culture
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Oct 11 2019

Immunohistochemistry (Frozen sections) abreview for Anti-FAM38A/PIEZO1 antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (Retina)
Permeabilization
No
Specification
Retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Marija Matejcic

Verified customer

Submitted Sep 14 2016

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-FAM38A/PIEZO1 antibody

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain (motor cortex))
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Specification
brain (motor cortex)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Patrick Ye

Verified customer

Submitted Aug 15 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FAM38A/PIEZO1 antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum slices)
Antigen retrieval step
None
Permeabilization
Yes - 10% BSA 0.5%Tx in PBS
Specification
Cerebellum slices
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Ms. Maria Velasco

Verified customer

Submitted Jun 23 2016

Western blot abreview for Anti-FAM38A/PIEZO1 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
30 µg
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Ms. Maria Velasco

Verified customer

Submitted Apr 08 2016

Question

Inquiry: Is the immunogenic peptide available for this product (ab128245)?

Read More
Answer

Thank you for your patience.

The immunogen peptide has been added to the catalog as ab133015.
https://www.abcam.com/index.html?datasheet=133015

The peptide is currently backordered, but if you place an order you should receive it within 1-2 weeks.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Is the immunogenic peptide available for this product (ab128245)? Thanks.

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Answer

Thank you for your patience. We are still looking into adding the immunogen to ourcatalogue and will have an answer for you early next week.

Read More

Question

Hi, we are looking to order one of these for IHC. This one (ab82336) says that its for WB but has 2 pictures that look like IHC. The other (ab128245) says IHC but does not have the pics. Could you please help us determine which one of these antibodies to get?

Read More
Answer

Thank you for your enquiry.

I would be happy to help you choose the most appropriate antibody for your IHC experiment. I just have a couple additional questions about your experiment:

1. Will you be using frozen sections or paraffin embedded sections?

2. What species of tissue will you be using?

Thanks in advance for the additional information!

Read More

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