Anti-FAM3C/ILEI antibody (ab72182)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-FAM3C/ILEI antibody
See all FAM3C/ILEI primary antibodies -
Description
Rabbit polyclonal to FAM3C/ILEI -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Chicken, Cow, Dog, Human, Monkey
Predicted to work with: Rat, Chimpanzee, Pufferfish, Opossum
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Immunogen
Synthetic peptide corresponding to Human FAM3C/ILEI.
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Positive control
- WB: Human, mouse and rat brain lysates. IHC-P: Human brain and skin tissue. Mouse brain tissue.
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General notes
This product was previously labelled as FAM3C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab72182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). | |
| IHC-P | Use a concentration of 5 - 10 µg/ml. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition. -
Tissue specificity
Present in most secretory epithelia (at protein level). -
Sequence similarities
Belongs to the FAM3 family. -
Cellular localization
Secreted. Cytoplasmic vesicle. Cytoplasmic in some cancer cells. - Information by UniProt
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Database links
- Entrez Gene: 417758 Chicken
- Entrez Gene: 615690 Cow
- Entrez Gene: 10447 Human
- Entrez Gene: 27999 Mouse
- Entrez Gene: 312159 Rat
- Omim: 608618 Human
- SwissProt: Q92520 Human
- SwissProt: Q91VU0 Mouse
see all -
Alternative names
- Fam3c antibody
- FAM3C_HUMAN antibody
- Family with sequence similarity 3 member C antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of mouse brain using ab72182 at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles
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Western blot - Anti-FAM3C/ILEI antibody (ab72182)All lanes : Anti-FAM3C/ILEI antibody (ab72182) at 0.5 µg/ml
Lane 1 : Human brain lysate
Lane 2 : Human brain lysate with immunising peptide
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)ab72182 at 5µg/ml staining FAM3C/ILEI in normal human brain by Immunohistochemistry using paraffin-embedded tissue. (Control rabbit Ig used for figure B).
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Western blot - Anti-FAM3C/ILEI antibody (ab72182)All lanes : Anti-FAM3C/ILEI antibody (ab72182) at 2 µg/ml
Lane 1 : Human testis tissue lysate with 5% non-fat milk in TBST
Lane 2 : Mouse testis tissue lysate with 5% non-fat milk in TBST
Secondary
All lanes : anti-rabbit HRP
Predicted band size: 25 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)ab72182 at 10µg/ml staining FAM3C/ILEI in human skin by Immunohistochemistry using paraffin-embedded tissue.
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Immunocytochemistry/ Immunofluorescence - Anti-FAM3C/ILEI antibody (ab72182)ICC/IF image of ab72182 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72182, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Yang W et al. FAM3C-YY1 axis is essential for TGFß-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1. J Cell Mol Med 23:3464-3475 (2019). Read more (PubMed: 30887707) »
- Woosley AN et al. TGFß promotes breast cancer stem cell self-renewal through an ILEI/LIFR signaling axis. Oncogene 38:3794-3811 (2019). Read more (PubMed: 30692635) »
