Anti-FAM3C/ILEI antibody (ab72182)
Key features and details
- Rabbit polyclonal to FAM3C/ILEI
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FAM3C/ILEI antibody
See all FAM3C/ILEI primary antibodies -
Description
Rabbit polyclonal to FAM3C/ILEI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanWB Human
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Immunogen
Synthetic peptide corresponding to Human FAM3C/ILEI.
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General notes
This product was previously labelled as FAM3C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Mouse
Human
|
| WB |
Human
|
| All applications |
Chimpanzee
Pufferfish
Opossum
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
|
| IHC-P |
Use a concentration of 5 - 10 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). |
|
IHC-P
Use a concentration of 5 - 10 µg/ml. |
Target
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Function
May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition. -
Tissue specificity
Present in most secretory epithelia (at protein level). -
Sequence similarities
Belongs to the FAM3 family. -
Cellular localization
Secreted. Cytoplasmic vesicle. Cytoplasmic in some cancer cells. - Information by UniProt
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Database links
- Entrez Gene: 10447 Human
- Entrez Gene: 27999 Mouse
- Entrez Gene: 312159 Rat
- Omim: 608618 Human
- SwissProt: Q92520 Human
- SwissProt: Q91VU0 Mouse
- SwissProt: Q810F4 Rat
- Unigene: 272375 Human
see all -
Alternative names
- Fam3c antibody
- FAM3C_HUMAN antibody
- Family with sequence similarity 3 member C antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)
IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of mouse brain using ab72182 at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles
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Western blot - Anti-FAM3C/ILEI antibody (ab72182)All lanes : Anti-FAM3C/ILEI antibody (ab72182) at 0.5 µg/ml
Lane 1 : Human brain lysate
Lane 2 : Human brain lysate with immunising peptide
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)ab72182 at 5µg/ml staining FAM3C/ILEI in normal human brain by Immunohistochemistry using paraffin-embedded tissue. (Control rabbit Ig used for figure B).
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Western blot - Anti-FAM3C/ILEI antibody (ab72182)All lanes : Anti-FAM3C/ILEI antibody (ab72182) at 2 µg/ml
Lane 1 : Human testis tissue lysate with 5% non-fat milk in TBST
Lane 2 : Mouse testis tissue lysate with 5% non-fat milk in TBST
Secondary
All lanes : anti-rabbit HRP
Predicted band size: 25 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)ab72182 at 10µg/ml staining FAM3C/ILEI in human skin by Immunohistochemistry using paraffin-embedded tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab72182 has been referenced in 6 publications.
- Yang W et al. FAM3C-YY1 axis is essential for TGFß-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1. J Cell Mol Med 23:3464-3475 (2019). PubMed: 30887707
- Woosley AN et al. TGFß promotes breast cancer stem cell self-renewal through an ILEI/LIFR signaling axis. Oncogene 38:3794-3811 (2019). PubMed: 30692635
- Noguchi K et al. Interleukin-like EMT inducer (ILEI) promotes melanoma invasiveness and is transcriptionally up-regulated by upstream stimulatory factor-1 (USF-1). J Biol Chem 293:11401-11414 (2018). PubMed: 29871931
- Noguchi K et al. Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines. PLoS One 12:e0177830 (2017). PubMed: 28545079
- Chen Z et al. FAM3C activates HSF1 to suppress hepatic gluconeogenesis and attenuate hyperglycemia of type 1 diabetic mice. Oncotarget 8:106038-106049 (2017). PubMed: 29285313
- Sun Y et al. The ubiquitin ligase UBE4A inhibits prostate cancer progression by targeting interleukin-like EMT inducer (ILEI). IUBMB Life 69:16-21 (2017). PubMed: 27862841