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    fam3cilei-antibody-ab72182.pdf

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Anti-FAM3C/ILEI antibody (ab72182)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)
  • Western blot - Anti-FAM3C/ILEI antibody (ab72182)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)
  • Western blot - Anti-FAM3C/ILEI antibody (ab72182)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)

Key features and details

  • Rabbit polyclonal to FAM3C/ILEI
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FAM3C/ILEI antibody
    See all FAM3C/ILEI primary antibodies
  • Description

    Rabbit polyclonal to FAM3C/ILEI
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human FAM3C/ILEI.

  • General notes

     This product was previously labelled as FAM3C

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Immunology
    • Secreted Molecules
    • Other secreted molecules

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human FAM3C/ILEI protein (ab151637)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab72182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
Human
WB
Human
All applications
Chimpanzee
Pufferfish
Opossum
Application Abreviews Notes
WB (2)
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P
Use a concentration of 5 - 10 µg/ml.
Notes
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P
Use a concentration of 5 - 10 µg/ml.

Target

  • Function

    May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.
  • Tissue specificity

    Present in most secretory epithelia (at protein level).
  • Sequence similarities

    Belongs to the FAM3 family.
  • Cellular localization

    Secreted. Cytoplasmic vesicle. Cytoplasmic in some cancer cells.
  • Target information above from: UniProt accession Q92520 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10447 Human
    • Entrez Gene: 27999 Mouse
    • Entrez Gene: 312159 Rat
    • Omim: 608618 Human
    • SwissProt: Q92520 Human
    • SwissProt: Q91VU0 Mouse
    • SwissProt: Q810F4 Rat
    • Unigene: 272375 Human
    • Unigene: 434053 Human
    • Unigene: 162738 Rat
    see all
  • Alternative names

    • Fam3c antibody
    • FAM3C_HUMAN antibody
    • Family with sequence similarity 3 member C antibody
    • GS3786 antibody
    • ILEI antibody
    • Interleukin-like EMT inducer antibody
    • Predicted osteoblast protein antibody
    • Protein FAM3C antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)

    IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of mouse brain using ab72182 at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles

  • Western blot - Anti-FAM3C/ILEI antibody (ab72182)
    Western blot - Anti-FAM3C/ILEI antibody (ab72182)
    All lanes : Anti-FAM3C/ILEI antibody (ab72182) at 0.5 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate with immunising peptide
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate

    Predicted band size: 25 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)

    ab72182 at 5µg/ml staining FAM3C/ILEI in normal human brain by Immunohistochemistry using paraffin-embedded tissue. (Control rabbit Ig used for figure B).

  • Western blot - Anti-FAM3C/ILEI antibody (ab72182)
    Western blot - Anti-FAM3C/ILEI antibody (ab72182)
    All lanes : Anti-FAM3C/ILEI antibody (ab72182) at 2 µg/ml

    Lane 1 : Human testis tissue lysate with 5% non-fat milk in TBST
    Lane 2 : Mouse testis tissue lysate with 5% non-fat milk in TBST

    Secondary
    All lanes : anti-rabbit HRP

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)

    ab72182 at 10µg/ml staining FAM3C/ILEI in human skin by Immunohistochemistry using paraffin-embedded tissue.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab72182? Please let us know so that we can cite the reference in this datasheet.

ab72182 has been referenced in 6 publications.

  • Yang W  et al. FAM3C-YY1 axis is essential for TGFß-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1. J Cell Mol Med 23:3464-3475 (2019). PubMed: 30887707
  • Woosley AN  et al. TGFß promotes breast cancer stem cell self-renewal through an ILEI/LIFR signaling axis. Oncogene 38:3794-3811 (2019). PubMed: 30692635
  • Noguchi K  et al. Interleukin-like EMT inducer (ILEI) promotes melanoma invasiveness and is transcriptionally up-regulated by upstream stimulatory factor-1 (USF-1). J Biol Chem 293:11401-11414 (2018). PubMed: 29871931
  • Noguchi K  et al. Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines. PLoS One 12:e0177830 (2017). PubMed: 28545079
  • Chen Z  et al. FAM3C activates HSF1 to suppress hepatic gluconeogenesis and attenuate hyperglycemia of type 1 diabetic mice. Oncotarget 8:106038-106049 (2017). PubMed: 29285313
  • Sun Y  et al. The ubiquitin ligase UBE4A inhibits prostate cancer progression by targeting interleukin-like EMT inducer (ILEI). IUBMB Life 69:16-21 (2017). PubMed: 27862841

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-FAM3C/ILEI antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Prostate)
Permeabilization
No
Specification
Prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

Submitted Jun 08 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-FAM3C/ILEI antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate)
Permeabilization
No
Specification
prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Dr. Olayinka Oloko

Verified customer

Submitted Dec 08 2011

Western blot abreview for Anti-FAM3C antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse mammary cell)
Loading amount
50 µg
Specification
Mouse mammary cell
Treatment
5ng/ml TGF beta for different time periods (hrs)
Gel Running Conditions
Reduced Denaturing (10.0% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Aug 31 2011

Immunocytochemistry abreview for Anti-FAM3C/ILEI antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (Prostate)
Permeabilization
Yes - Triton X-100
Specification
Prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Methanol
Read More

Dr. Olayinka Oloko

Verified customer

Submitted May 27 2011

Western blot abreview for Anti-FAM3C antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate)
Loading amount
25 µg
Specification
Prostate
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Read More

Dr. Olayinka Oloko

Verified customer

Submitted Apr 01 2011

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