Product nameAnti-FAM49B antibody
DescriptionRabbit polyclonal to FAM49B
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to amino acids 2-134 of Human FAM49B (Q9NUQ9).
- RT4, U-251 MG and Human Tonsil lysate; Human kidney tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesBelongs to the FAM49 family.
- Information by UniProt
- BM 009 antibody
- FA49B_HUMAN antibody
- Fam49b antibody
All lanes : Anti-FAM49B antibody (ab121299) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : Human Plasma lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab121299 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121299 at 1/100 dilution staining FAM49B in Paraffin-emebedded Human kidney tissue by Immunohistochemistry.
ab121299 has not yet been referenced specifically in any publications.