Overview

  • Product name

  • Description

    Rabbit polyclonal to FAM50B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Recombinant fragment corresponding to Human FAM50B aa 104-133.
    Sequence:

    QREQEQRRERKRKISCLSFALDDLDDQADA


    Database link: Q9Y247

  • Positive control

    • HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Tissue specificity

    Widely expressed. Mostly abundant in testis and adult and fetal brain.
  • Sequence similarities

    Belongs to the FAM50 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • D0H6S2654E antibody
    • D6S2654E antibody
    • DNA segment, human D6S2654E antibody
    • FA50B_HUMAN antibody
    • FAM50B antibody
    • Family with sequence similarity 50, member B antibody
    • Protein FAM50B antibody
    • Protein XAP 5 like antibody
    • Protein XAP-5-like antibody
    • X5L antibody
    • XAP5 like protein antibody
    see all

Images

  • Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized HeLa cells labeling FAM50B with ab221467 at 4 µg/ml.  Antibody staining is shown in green.  

References

ab221467 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma cells)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
100000 cells
Specification
Melanoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Nicola Thompson

Verified customer

Submitted Feb 06 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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