Anti-FAM63B antibody (ab151122)
Key features and details
- Rabbit polyclonal to FAM63B
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAM63B antibody -
Description
Rabbit polyclonal to FAM63B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to amino acids 165-252 of Human FAM63B.
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Positive control
- IHC-P: Human prostate, placenta, testis and liver tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| Notes |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins. May play a regulatory role at the level of protein turnover. -
Sequence similarities
Belongs to the peptidase MINDY family. FAM63 subfamily. - Information by UniProt
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Database links
- Entrez Gene: 54629 Human
- Entrez Gene: 235461 Mouse
- Entrez Gene: 363089 Rat
- SwissProt: Q8NBR6 Human
- SwissProt: Q6PDI6 Mouse
- Unigene: 591122 Human
- Unigene: 595286 Human
- Unigene: 170855 Mouse
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Alternative names
- Deubiquitinating enzyme MINDY-2 antibody
- FA63B_HUMAN antibody
- FAM63B antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (ab151122)
Paraffin embedded human prostate tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (ab151122)Paraffin embedded human placenta tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (ab151122)Paraffin embedded human testis tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-FAM63B antibody (ab151122)Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (ab151122)Paraffin embedded human liver tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab151122 has not yet been referenced specifically in any publications.
