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Rabbit polyclonal to FAM82A2
Predicted to work with:
Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 420 - 470 of Human FAM82A2 (NP_060615.1).
HeLa whole cell lysates and 293T cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab100803 is affinity purified using an epitope specific to FAM82A2 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
Use at 2-5 µg/mg of lysate.
FAM82A2, or family with sequence similarity 82, member C, may participate in differentiation and apoptosis of keratinocytes. Overexpression of FAM82A2 induces apoptosis.
Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Nucleus. Note=In interphase localizes in the cytoplasm, and during mitosis localizes to the spindle microtubules and spindle poles.
Cerebral protein 10 antibody
FAM82C, formerly antibody
Western blot - Anti-FAM82A2 antibody (ab100803)
All lanes : Anti-FAM82A2 antibody (ab100803) at 0.1 µg/ml Lane 1 : HeLa whole cell lysates at 50 µg Lane 2 : HeLa whole cell lysates at 15 µg Lane 3 : HeLa whole cell lysates at 5 µg Lane 4 : 293T cell lysates at 50 µg Developed using the ECL technique. Predicted band size: 52 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-FAM82A2 antibody (ab100803)
Detection of Human FAM82A2 by western blot of immunoprecipitates of HeLa whole cell lysates (at 1 mg for IP, 20% of IP loaded) using ab100803 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent blotting.
Detection by chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"