Key features and details
- Rabbit polyclonal to FAM83G/PAWS1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAM83G/PAWS1 antibody
See all FAM83G/PAWS1 primary antibodies
DescriptionRabbit polyclonal to FAM83G/PAWS1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- RT4, U-251 MG, Human Liver and Human Tonsil lysates, Human oral mucosa tissue
This product was previously labelled as FAM83G
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the FAM83 family.
- Information by UniProt
- FA83G_HUMAN antibody
- Fam83g antibody
- Family with sequence similarity 83, member G antibody
- Protein FAM83G antibody
ab121750 at 1/100 dilution staining FAM83G/PAWS1 in Paraffin-emebedded Human oral mucosa tissue by Immunohistochemistry.
Immunocytochemistry/Immunofluorescence analysis of U-251 MG cells labelling FAM83G/PAWS1 with ab121750 shows positivity in the cytoplasm.
All lanes : Anti-FAM83G/PAWS1 antibody (ab121750) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
10-20% SDS PAGE
ab121750 has been referenced in 3 publications.
- Wu KZL et al. Pathogenic FAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1a association and attenuate Wnt signalling. Wellcome Open Res 4:133 (2019). PubMed: 31656861
- Maruthappu T et al. Recessive Mutation in FAM83G Associated with Palmoplantar Keratoderma and Exuberant Scalp Hair. J Invest Dermatol 138:984-987 (2018). IHC-P ; Human . PubMed: 29138053
- Bozatzi P et al. PAWS1 controls Wnt signalling through association with casein kinase 1a. EMBO Rep 19:N/A (2018). ICC/IF . PubMed: 29514862