Product nameAnti-FAM83G/PAWS1 antibody
See all FAM83G/PAWS1 primary antibodies
DescriptionRabbit polyclonal to FAM83G/PAWS1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- RT4, U-251 MG, Human Liver and Human Tonsil lysates, Human oral mucosa tissue
This product was previously labelled as FAM83G
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
|WB||1/250 - 1/500.|
Sequence similaritiesBelongs to the FAM83 family.
- Information by UniProt
- FA83G_HUMAN antibody
- Fam83g antibody
- Family with sequence similarity 83, member G antibody
- Protein FAM83G antibody
ab121750 at 1/100 dilution staining FAM83G/PAWS1 in Paraffin-emebedded Human oral mucosa tissue by Immunohistochemistry.
Immunocytochemistry/Immunofluorescence analysis of U-251 MG cells labelling FAM83G/PAWS1 with ab121750 shows positivity in the cytoplasm.
All lanes : Anti-FAM83G/PAWS1 antibody (ab121750) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
10-20% SDS PAGE
This product has been referenced in:
- Maruthappu T et al. Recessive Mutation in FAM83G Associated with Palmoplantar Keratoderma and Exuberant Scalp Hair. J Invest Dermatol 138:984-987 (2018). IHC-P ; Human . Read more (PubMed: 29138053) »
- Bozatzi P et al. PAWS1 controls Wnt signalling through association with casein kinase 1a. EMBO Rep 19:N/A (2018). ICC/IF . Read more (PubMed: 29514862) »