Overview

  • Product name

    Anti-FAM91A1 antibody
  • Description

    Rabbit polyclonal to FAM91A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residue 300 and 350 of human FAM91A1 (NP_659400.2)

  • Positive control

    • Whole cell lysate from HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab81618 was affinity purified using an epitope specific to FAM91A1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notes
    IP: Use at 2 - 5 µg/mg of lysate.

    Is unsuitable for WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Detection of FAM91A1 by Western Blot of immunoprecipitates in whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab81618 was used for IP at 3 µg/mg lysate (lane 1). Lane 2 represents control IgG. Detection was by chemiluminescence with an exposure time of 3 seconds.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hap1 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycine)
    Loading amount
    5 µg
    Specification
    Hap1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Jun 18 2019

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