Product nameAnti-FAM96B antibody
See all FAM96B primary antibodies
DescriptionRabbit polyclonal to FAM96B
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse gonadal tissue; MCF7, HeLa, K562 and A375 whole cell lysates. IHC-P: Human kidney tissue. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionComponent of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD.
Sequence similaritiesBelongs to the MIP18 family.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle.
- Information by UniProt
- CGI 128 antibody
- FAM96B antibody
- Family with sequence similarity 96, member B antibody
All lanes : Anti-TRIM10 antibody (ab230409) at 1/10000 dilution
Lane 1 : Mouse gonadal tissue lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : A-375 (human malignant melanoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 18 kDa
Paraffin-embedded human kidney tissue stained for FAM96B using ab230410 at 1/100 dilution in immunochemical analysis.
FAM96B was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg of ab230410. Western blot was performed from the immunoprecipitate using ab230410 and an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at 1/50,000 dilution.
Lane 1: 1 µg of rabbit monoclonal IgG instead of ab230410 in HeLa whole cell lysate.
Lane 2: ab230410 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (input).
ab230410 has not yet been referenced specifically in any publications.