Key features and details
- Rabbit polyclonal to FAM96B
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FAM96B antibody
See all FAM96B primary antibodies
DescriptionRabbit polyclonal to FAM96B
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse gonadal tissue; MCF7, HeLa, K562 and A375 whole cell lysates. IHC-P: Human kidney tissue. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionComponent of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD.
Sequence similaritiesBelongs to the MIP18 family.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle.
- Information by UniProt
- CGI 128 antibody
- FAM96B antibody
- Family with sequence similarity 96, member B antibody
All lanes : Anti-TRIM10 antibody (ab230409) at 1/10000 dilution
Lane 1 : Mouse gonadal tissue lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : A-375 (human malignant melanoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 18 kDa
Paraffin-embedded human kidney tissue stained for FAM96B using ab230410 at 1/100 dilution in immunochemical analysis.
FAM96B was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg of ab230410. Western blot was performed from the immunoprecipitate using ab230410 and an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at 1/50,000 dilution.
Lane 1: 1 µg of rabbit monoclonal IgG instead of ab230410 in HeLa whole cell lysate.
Lane 2: ab230410 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (input).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230410 has not yet been referenced specifically in any publications.