RecombinantRabMAb

Recombinant Anti-FAM96B antibody [EPR10178] (ab166607)

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Western blot - Anti-FAM96B antibody [EPR10178] (ab166607)
  • Immunoprecipitation - Anti-FAM96B antibody [EPR10178] (ab166607)
  • Flow Cytometry - Anti-FAM96B antibody [EPR10178] (ab166607)
  • Anti-FAM96B antibody [EPR10178] (ab166607)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10178] to FAM96B
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FAM96B antibody [EPR10178]
    See all FAM96B primary antibodies

  • Description

    Rabbit monoclonal [EPR10178] to FAM96B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human FAM96B (UniProt ID: Q9Y3D0).

  • Positive control

    • HeLa, K562 and Ramos cell lysates, k562 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10178

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.

    • Target

    • Function

      Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD.

    • Sequence similarities

      Belongs to the MIP18 family.

    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > spindle.
    • Information by UniProt

    • Database links

      • Alternative names

        • CGI 128 antibody
        • FAM96B antibody
        • Family with sequence similarity 96, member B antibody
        • HSPC118 antibody
        • Hypothetical protein LOC51647 antibody
        • MIP18 antibody
        • MIP18_HUMAN antibody
        • Mitotic spindle-associated MMXD complex subunit MIP18 antibody
        • MSS19-interacting protein of 18 kDa antibody
        • Protein FAM96B antibody
        see all

      Images

      • Western blot - Anti-FAM96B antibody [EPR10178] (ab166607)
        Western blot - Anti-FAM96B antibody [EPR10178] (ab166607)
        All lanes : Anti-FAM96B antibody [EPR10178] (ab166607) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : Ramos cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 18 kDa
        Observed band size: 18 kDa

      • Immunoprecipitation - Anti-FAM96B antibody [EPR10178] (ab166607)
        Immunoprecipitation - Anti-FAM96B antibody [EPR10178] (ab166607)
        Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166607 and HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG. Predicted band size : 18 kDa.
      • Flow Cytometry - Anti-FAM96B antibody [EPR10178] (ab166607)
        Flow Cytometry - Anti-FAM96B antibody [EPR10178] (ab166607)
        Flow Cytometrical analysis of permeabilized K562 cells with ab166607 antibody at 1/100 (red) compared to a rabbit IgG negative (green).
      • Anti-FAM96B antibody [EPR10178] (ab166607)
        Anti-FAM96B antibody [EPR10178] (ab166607)

      References (2)

      Publishing research using ab166607? Please let us know so that we can cite the reference in this datasheet.

      ab166607 has been referenced in 2 publications.

      • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). PubMed: 30765471
      • Kim KS  et al. Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. Hum Mol Genet 27:837-852 (2018). PubMed: 29309586

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