Recombinant
RabMAb

Recombinant Anti-FAM96B antibody [EPR10178] (ab166607)

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Overview

  • Product name

    Anti-FAM96B antibody [EPR10178]
    See all FAM96B primary antibodies

  • Description

    Rabbit monoclonal [EPR10178] to FAM96B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human FAM96B (UniProt ID: Q9Y3D0).

  • Positive control

    • HeLa, K562 and Ramos cell lysates, k562 cells

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10178

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.

    • Target

    • Function

      Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD.

    • Sequence similarities

      Belongs to the MIP18 family.

    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > spindle.
    • Information by UniProt

    • Database links

      • Alternative names

        • CGI 128 antibody
        • FAM96B antibody
        • Family with sequence similarity 96, member B antibody
        • HSPC118 antibody
        • Hypothetical protein LOC51647 antibody
        • MIP18 antibody
        • MIP18_HUMAN antibody
        • Mitotic spindle-associated MMXD complex subunit MIP18 antibody
        • MSS19-interacting protein of 18 kDa antibody
        • Protein FAM96B antibody
        see all

      Images

      • Western blot - Anti-FAM96B antibody [EPR10178] (ab166607)
        Western blot - Anti-FAM96B antibody [EPR10178] (ab166607)
        All lanes : Anti-FAM96B antibody [EPR10178] (ab166607) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : Ramos cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 18 kDa
        Observed band size: 18 kDa

      • Immunoprecipitation - Anti-FAM96B antibody [EPR10178] (ab166607)
        Immunoprecipitation - Anti-FAM96B antibody [EPR10178] (ab166607)
        Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166607 and HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG. Predicted band size : 18 kDa.
      • Flow Cytometry - Anti-FAM96B antibody [EPR10178] (ab166607)
        Flow Cytometry - Anti-FAM96B antibody [EPR10178] (ab166607)
        Flow Cytometrical analysis of permeabilized K562 cells with ab166607 antibody at 1/100 (red) compared to a rabbit IgG negative (green).

      References

      This product has been referenced in:

      • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). Read more (PubMed: 30765471) »
      • Kim KS  et al. Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. Hum Mol Genet 27:837-852 (2018). Read more (PubMed: 29309586) »
      See all 2 Publications for this product

      Publishing research using ab166607? Please let us know so that we can cite the reference in this datasheet.

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